LOCUS       sequence-79421-e       13120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The oriP/EBV nuclear antigen (EBNA)-1 elements of Epstein-Barr virus
            is fused in frame with the luciferase in the herpes simplex virus 
            type-1 amplicon vector, provides prolonged transgene expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13120)
  AUTHORS   Sia KC, Chong WK, Ho IA, Yulyana Y, Endaya B, Huynh H, Lam PY
  TITLE     Hybrid herpes simplex virus/Epstein-Barr virus amplicon viral 
            vectors confer enhanced transgene expression in primary human tumors
            and human bone marrow-derived mesenchymal stem cells.
  JOURNAL   J Gene Med. 2010 Oct;12(10):848-58. doi: 10.1002/jgm.1506.
  PUBMED    20963807
REFERENCE   2  (bases 1 to 13120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/jgm"; 
            journalName: "J Gene Med"; date: "2010-10"; volume: "12"; issue: 
            "10"; pages: "848-58"
COMMENT     Sequence Label: pHCAG-L2EOP
FEATURES             Location/Qualifiers
     source          1..13120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(362..380)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        475..579
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(580..648,649..1440)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(798..817)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1611..2199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2100..2119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2353..2370
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2652..2674
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     regulatory      3753..3762
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3759..3761,3762..3764,3765..4478)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3759..3761,3762..3764,3765..4475)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3804..3825)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4065..4084)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4412..4433
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4486..4620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        4851..5230
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5232..5509
                     /label=chicken beta-actin promoter
     primer_bind     6450..6472
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      6574..6583
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6580..8229
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(6646..6664)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             8230..8295
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     rep_origin      9854..11643
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     polyA_signal    12426..12560
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ggggaggagc ggggggagga gcggggggag gagcgggggg aggagcggcc agaccccgaa
       61 aacgggcccc ccccaaaaca caccccccgg gggtcgcgcg cggcccttta aagcggcggc
      121 ggcgggcagc ccgggccccc cgcggccgag actagcgagt tagacaggca agcactactc
      181 gcctctgcac gcacatgctt gcctgtcaaa ctctaccacc ccggcacgct ctctgtctcc
      241 atggcccgcc gccgccgcca tcgcggcccc cgccgccccc ggccgcccgg gcccacgggc
      301 gccgtcccaa ccgcacagtc ccaggtaacg actctaggaa ttaattcttg aagacgaaag
      361 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagggt
      421 cgaccggtcg accggtcgac cggtcgacca ggtggcactt ttcggggaaa tgtgcgcgga
      481 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      541 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
      601 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
      661 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
      721 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
      781 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
      841 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
      901 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
      961 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     1021 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     1081 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     1141 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     1201 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     1261 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     1321 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     1381 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     1441 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     1501 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     1561 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1621 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1681 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1741 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     1801 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1861 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1921 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1981 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2041 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2101 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2161 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2221 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2281 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     2341 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     2401 ctccttacgc atctgtgcgg tatttcacac cgcataggtc gaccggtcga ccggtcgacc
     2461 ggtcgaccgg tcgaccatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     2521 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     2581 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     2641 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     2701 gaggcagcat gaattattcc attatgcacg accccgcccc gacgccggca cgccgggggc
     2761 ccgtggccgc ggcccgttgg tcgaaccccc ggccccgccc atccgcgcca tctgccatgg
     2821 gcggggcgcg agggcgggtg ggtccgcgcc ccgccccgca tggcatctca ttaccgcccg
     2881 atccggcggt ttccgcttcc gttccgcatg ctaacgagga acaggcaggg ggcggggccc
     2941 gggccccgac ttcccggttc ggcggtaatg agatacgagc cccgcgcgcc cgttggccgt
     3001 ccccgggccc cccggtcccg cccgccggac gccgggacca acgggacggc gggcggccca
     3061 agggccgccc gccttgccgc ccccccattg gccggcgggc gggaccgccc caagggggcg
     3121 gggccgccgg gtaaaagaag tgagaacgcg aagcgttcgc acttcgtccc aatatatata
     3181 tattattagg gcgaagtgcg agcactggcg ccgtgcccga ctccgcgccg gccccggggg
     3241 cgggcccggg cggcgggggg cgggtctctc cggcgcacat aaaggcccgg cgcgaccgac
     3301 gcccgcagac ggcgccggcc acgaacgacg ggagcggctg cggagcacgc ggaccgggag
     3361 cgggagtcgc agagggccgt cggagcggac ggcgtcggca tcgcgacgcc ccggctcggg
     3421 atcgggatcg catcggaaag ggacacgcgg acgcgggggg gaaagacccg cccaccccac
     3481 ccacgaaaca caggggacgc accccggggg cctccgacga cagaaaccca ccggtccgcc
     3541 ttttttgcac gggtaagcac cttgggtggg cggaggaggg ggggacgcgg gggcggagga
     3601 ggggggacgc gggggcggag gaggggggac gcgggggcgg aggagggggg acgcgggggc
     3661 ggaggagggg ggacgcgggg gcggaggagg gggctcaccc gcgttcgtgc cttcccgcag
     3721 gaggaacgtc ctcgtcgata agctgatcca tcgccaccat ggtgagcaag ggcgaggagc
     3781 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3841 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     3901 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     3961 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     4021 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     4081 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     4141 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     4201 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     4261 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     4321 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     4381 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     4441 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccaactt gtttattgca
     4501 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4561 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     4621 ggtttgaaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     4681 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     4741 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     4801 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
     4861 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     4921 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     4981 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     5041 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     5101 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     5161 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     5221 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     5281 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     5341 gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag
     5401 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
     5461 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
     5521 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
     5581 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
     5641 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
     5701 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
     5761 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
     5821 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc
     5881 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     5941 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg
     6001 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct
     6061 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
     6121 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
     6181 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     6241 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
     6301 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
     6361 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     6421 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     6481 tagcgtttaa acttaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt
     6541 ctgcagatat ccagcacagt ggcggccgct cgagccacca tggaagacgc caaaaacata
     6601 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat
     6661 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
     6721 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg
     6781 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
     6841 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
     6901 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc
     6961 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa
     7021 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
     7081 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat
     7141 agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt
     7201 gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt
     7261 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
     7321 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
     7381 tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg
     7441 gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct
     7501 aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt
     7561 gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca
     7621 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
     7681 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga
     7741 ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg
     7801 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
     7861 gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat
     7921 caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca
     7981 ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
     8041 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
     8101 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
     8161 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
     8221 atcgccgtgg tgaaacagac tttgaatttt gaccttctca agttggcggg agacgtcgag
     8281 tccaaccctg ggcccatcat gtctgacgag gggccaggta caggacctgg aaatggccta
     8341 ggagagaagg gagacacatc tggaccagaa ggctccggcg gcagtggacc tcaaagaaga
     8401 gggggtgata accatggacg aggacgggga agaggacgag gacgaggagg cggaagacca
     8461 ggagccccgg gcggctcagg atcagggcca agacatagag atggtgtccg gagaccccaa
     8521 aaacgtccaa gttgcattgg ctgcaaaggg acccacggtg gaacaggagc aggagcagga
     8581 gcgggagggg caggagcagg aggtggaggc cggggtcgag gaggcagtgg aggccggggt
     8641 cgaggaggta gtggaggccg gggtcgagga ggtagtggag gccgccgggg tagaggacgt
     8701 gaaagagcca gggggggaag tcgtgaaaga gccaggggga gaggtcgtgg acgtggagaa
     8761 aagaggccca ggagtcccag tagtcagtca tcatcatccg ggtctccacc gcgcaggccc
     8821 cctccaggta gaaggccatt tttccaccct gtaggggaag ccgattattt tgaataccac
     8881 caagaaggtg gcccagatgg tgagcctgac gtgcccccgg gagcgataga gcagggcccc
     8941 gcagatgacc caggagaagg cccaagcact ggaccccggg gtcagggtga tggaggcagg
     9001 cgcaaaaaag gagggtggtt tggaaagcat cgtggtcaag gaggttccaa cccgaaattt
     9061 gagaacattg cagaaggttt aagagctctc ctggctagga gtcacgtaga aaggactacc
     9121 gacgaaggaa cttgggtcgc cggtgtgttc gtatatggag gtagtaagac ctccctttac
     9181 aacctaaggc gaggaactgc ccttgctatt ccacaatgtc gtcttacacc attgagtcgt
     9241 ctcccctttg gaatggcccc tggacccggc ccacaacctg gcccgctaag ggagtccatt
     9301 gtctgttatt tcatggtctt tttacaaact catatatttg ctgaggtttt gaaggatgcg
     9361 attaaggacc ttgttatgac aaagcccgct cctacctgca atatcagggt gactgtgtgc
     9421 agctttgacg atggagtaga tttgcctccc tggtttccac ctatggtgga aggggctgcc
     9481 gcggagggtg atgacggaga tgacggagat gaaggaggtg atggagatga gggtgaggaa
     9541 gggcaggagt gatgtaactt gttaggagac gccctcaatc gtattaaaag ccgtgtattc
     9601 ccccgcacta aagaataaat ccccagtaga catcatgcgt gctgttggtg tatttctggc
     9661 catctgtctt gtcaccattt tcgtcctccc aacatggggc aattgggcat acccatgttg
     9721 tcacgtcact cagctccgcg ctcaacacct tctcgcgttg gaaaacatta gcgacattta
     9781 cctggtgagc aatcagacat gcgacggctt tagcctggcc tccttaaatt cacctaagaa
     9841 tgggagcaac cagcaggaaa aggacaagca gcgaaaattc acgccccctt gggaggtggc
     9901 ggcatatgca aaggatagca ctcccactct actactgggt atcatatgct gactgtatat
     9961 gcatgaggat agcatatgct acccggatac agattaggat agcatatact acccagatat
    10021 agattaggat agcatatgct acccagatat agattaggat agcctatgct acccagatat
    10081 aaattaggat agcatatact acccagatat agattaggat agcatatgct acccagatat
    10141 agattaggat agcctatgct acccagatat agattaggat agcatatgct acccagatat
    10201 agattaggat agcatatgct atccagatat ttgggtagta tatgctaccc agatataaat
    10261 taggatagca tatactaccc taatctctat taggatagca tatgctaccc ggatacagat
    10321 taggatagca tatactaccc agatatagat taggatagca tatgctaccc agatatagat
    10381 taggatagcc tatgctaccc agatataaat taggatagca tatactaccc agatatagat
    10441 taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc agatatagat
    10501 taggatagca tatgctatcc agatatttgg gtagtatatg ctacccatgg caacattagc
    10561 ccaccgtgct ctcagcgacc tcgtgaatat gaggaccaac aaccctgtgc ttggcgctca
    10621 ggcgcaagtg tgtgtaattt gtcctccaga tcgcagcaat cgcgccccta tcttggcccg
    10681 cccacctact tatgcaggta ttccccgggg tgccattagt ggttttgtgg gcaagtggtt
    10741 tgaccgcagt ggttagcggg gttacaatca gccaagttat tacaccctta ttttacagtc
    10801 caaaaccgca gggcggcgtg tgggggctga cgcgtgcccc cactccacaa tttcaaaaaa
    10861 aagagtggcc acttgtcttt gtttatgggc cccattggcg tggagccccg tttaattttc
    10921 gggggtgtta gagacaacca gtggagtccg ctgctgtcgg cgtccactct ctttcccctt
    10981 gttacaaata gagtgtaaca acatggttca cctgtcttgg tccctgcctg ggacacatct
    11041 taataacccc agtatcatat tgcactagga ttatgtgttg cccatagcca taaattcgtg
    11101 tgagatggac atccagtctt tacggcttgt ccccacccca tggatttcta ttgttaaaga
    11161 tattcagaat gtttcattcc tacactagta tttattgccc aaggggtttg tgagggttat
    11221 attggtgtca tagcacaatg ccaccactga accccccgtc caaattttat tctgggggcg
    11281 tcacctgaaa ccttgttttc gagcacctca catacacctt actgttcaca actcagcagt
    11341 tattctatta gctaaacgaa ggagaatgaa gaagcaggcg aagattcagg agagttcact
    11401 gcccgctcct tgatcttcag ccactgccct tgtgactaaa atggttcact accctcgtgg
    11461 aatcctgacc ccatgtaaat aaaaccgtga cagctcatgg ggtgggagat atcgctgttc
    11521 cttaggaccc ttttactaac cctaattcga tagcatatgc ttcccgttgg gtaacatatg
    11581 ctattgaatt agggttagtc tggatagtat atactactac ccgggaagca tatgctaccc
    11641 gtttagggtt aacaaggggg ccttataaac actattgcta atgccctctt gagggtccgc
    11701 ttatcggtag ctacacaggc ccctctgatt gacgttggtg tagcctcccg tagtcttcct
    11761 gggcccctgg gaggtacatg tcccccagca ttggtgtaag agcttcagcc aagagttaca
    11821 cataaagggc tagctcgaca tgataagata cattgatgag tttggacaaa ccacaactag
    11881 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtgaa
    11941 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
    12001 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
    12061 aagtaaaacc tctacaaatg tggtagatcc atttaaatgt taattaaatt ccagctgagc
    12121 gccggtcgct accattacca gttggtctgg tgtcaaaaat aataataacc gggcaggcca
    12181 tgtctgcccg tatttcgcgt aaggaaatcc attatgtact atttaaaaaa cacaaacttt
    12241 tggatgttcg gtttattctt tttcttttac ttttttatca tgggagccta cttcccgttt
    12301 ttcccgattt ggctacatga catcaaccat atcagcaaaa gtgatacggg tattattttt
    12361 gccgctattt ctctgttctc gctattattc caaccgctgt ttggtctgct ttctgacaaa
    12421 ctcggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    12481 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    12541 tatcttatca tgtctggatc tctgacctga gattggcggc actgaggtag agatgcccga
    12601 acccccccga gggagcgcgg gacgcggccg gggagggctg gggccgggga gggctggggc
    12661 cggggagggc tggggccggg gagggctggg gccggggagg gctggggccg gggagggctg
    12721 gggctgggga ggggcggtgg tgtgtagcag gagcggtgtg ttgcgccggg gtacgtctgg
    12781 aggagcggga ggtgcgcggt gacgtgtgga tgaggaacag gagttgttgc gcggtgagtt
    12841 gtcgctgtga gttgtgttgt tgggcaggtg tggtggatga cgtgacgtgt gacgtgcgga
    12901 gtgcgccgtg ctctgttggt ttcacctgtg gcagcccggg ccccccgcgg gcgcgcgcgc
    12961 gcgcaaaaaa ggcgggcggc ggtccgggcg gcgtgcgcgc gcgcggcggg cgtggggggc
    13021 ggggccgcgg gagcgggggg aggagcgggg ggaggagcgg ggggaggagc ggggggagga
    13081 gcggggggag gagcgggggg aggagcgggg ggaggagcgg
//
