LOCUS       sequence-79456-e         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NS module plasmid for Cermak, et al., 2011 TALEN kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  TITLE     module plasmid for Cermak, et al., 2011 TALEN kit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pNS7
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(214..231)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(344..349,350..366,367..372)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(772..791)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
       61 ctatggaaaa acgccagcaa cagcggcctt tttacggttc ctggcctttc gttttatttg
      121 atgcctggca gttccctact ctcgcgttct gcattaatga atcggccaac gcgcggggag
      181 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      241 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      301 atcaggggat aacgcaggaa agaacatgaa ttaattctca tgtttgacag cttatcatcg
      361 attagcttta atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta
      421 tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca
      481 taggcttggt tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca
      541 tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac
      601 ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac
      661 ttggagccac tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg attctctacg
      721 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
      781 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
      841 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttacatg
      901 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
      961 tgcaggagtc gcataaggga gagc
//
