LOCUS       sequence-79478-e        1497 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses CD4 with C-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Raissi AJ, Staudenmaier EK, David S, Hu L, Paradis S
  TITLE     Sema4D localizes to synapses and regulates GABAergic synapse 
            development as a membrane-bound molecule in the mammalian 
            hippocampus.
  JOURNAL   Mol Cell Neurosci. 2013 Nov;57:23-32. doi: 
            10.1016/j.mcn.2013.08.004. Epub 2013 Sep 10.
  PUBMED    24036351
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2013-11"; volume: "57:23-32. doi"; pages: " 
            10.1016/j.mcn.2013.08.004. Epub 2013 Sep 10"
COMMENT     Sequence Label: pCMV-CD4-FLAG
FEATURES             Location/Qualifiers
     source          1..1497
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1471..1494
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggctggga ttttctattt catcctcttt tcgtttctct ttggaatttg cgacgctgtc
       61 accggttcta gagggcccac catggcatca atgcagaagc tgatctcaga ggaggacctg
      121 cttatggcca tggaggcccg aattcggtcg accgagatct taaagaaagt ggtgctgggc
      181 aaaaaagggg atacagtgga actgacctgt acagcttccc agaagaagag catacaattc
      241 cactggaaaa actccaacca gataaagatt ctgggaattc agggctcctt cttaactaaa
      301 ggtccatcca agctgaatga tcgcgctgac tcaagaagaa gcctttggga ccaaggaaac
      361 ttccccctga tcatcaagaa tcttaagata gaagactcag atacttacat ctgtgaagtg
      421 gaggaccaga aggaggaggt gcaattgcta gtgttcggat tgactgccaa ctctgacacc
      481 cacctgcttc aggggcagag cctgaccctg accttggaga gcccccctgg tagtagcccc
      541 tcagtgcaat gtaggagtcc aaggggtaaa aacatacagg gggggaagac cctctccgtg
      601 tctcagctgg agctccagga tagtggcacc tggacatgca ctgtcttgca gaaccagaag
      661 aaggtggagt tcaaaataga catcgtggtg ctagctttcc agaaggcctc cagcatagtc
      721 tataagaaag agggggaaca ggtggagttc tccttcccac tcgcctttac agttgaaaag
      781 ctgacgggca gtggcgagct gtggtggcag gcggagaggg cttcctcctc caagtcttgg
      841 atcacctttg acctgaagaa caaggaagtg tctgtaaaac gggttaccca ggaccctaag
      901 ctccagatgg gcaagaagct cccgctccac ctcaccctgc cccaggcctt gcctcagtat
      961 gctggctctg gaaacctcac cctggccctt gaagcgaaaa caggaaagtt gcatcaggaa
     1021 gtgaacctgg tggtgatgag agccactcag ctccagaaaa atttgacctg tgaggtgtgg
     1081 ggacccacct cccctaagct gatgctgagc ttgaaactgg agaacaagga ggcaaaggtc
     1141 tcgaagcggg agaaggcggt gtgggtgctg aaccctgagg cggggatgtg gcagtgtctg
     1201 ctgagtgact cgggacaggt cctgctggaa tccaacatcg aggttctgcc cacatggtcc
     1261 accccggtgc agccaatggc cctgattgtg ctggggggcg tcgccggcct cctgcttttc
     1321 attgggctag gcatcttctt ctgtgtcagg tgccggcacc gaaggcgcca agcagagcgg
     1381 atgtctcaga tcaagagact cctcagtgag aagaagacct gccagtgccc tcaccggttt
     1441 cagaagacat gtagccccat tggagcggcc gactacaagg acgacgatga caagtga
//
