LOCUS       sequence-79481-e        2244 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses myc-tagged Sema4D/CD4 non-cleavable chimera with 
            extracellular Sema4D, intracellular CD4 in lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Raissi AJ, Staudenmaier EK, David S, Hu L, Paradis S
  TITLE     Sema4D localizes to synapses and regulates GABAergic synapse 
            development as a membrane-bound molecule in the mammalian 
            hippocampus.
  JOURNAL   Mol Cell Neurosci. 2013 Nov;57:23-32. doi: 
            10.1016/j.mcn.2013.08.004. Epub 2013 Sep 10.
  PUBMED    24036351
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2013-11"; volume: "57:23-32. doi"; pages: " 
            10.1016/j.mcn.2013.08.004. Epub 2013 Sep 10"
COMMENT     Sequence Label: FRIG-myc-Sema4D-CD4
FEATURES             Location/Qualifiers
     source          1..2244
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2218..2241
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggctggga ttttctattt catcctcttt tcgtttctct ttggaatttg cgacgctgtc
       61 accggttcta gagggcccac catggcatca atgcagaagc tgatctcaga ggaggacctg
      121 cttatggcca tggaggcccg aattcggtcg accgagatct ctcgaggttc ggccttgctg
      181 atgagtgagg acaaagacac tctgtatgta ggcgcccggg aagcagtctt tgcagtgaat
      241 gcgctgaaca tctctgagaa gcaacatgag gtatattgga aggtctctga agacaaaaaa
      301 tccaagtgtg cagagaaggg gaaatcaaag cagacggagt gccttaacta cattcgagta
      361 ctacagccac taagcagcac ttccctctat gtgtgtggga ccaatgcgtt ccagcccacc
      421 tgtgaccacc tgaacttgac atccttcaag tttctgggga aaagtgaaga tggcaaagga
      481 agatgcccct tcgaccccgc ccacagctac acatcagtca tggttggggg cgagctctac
      541 tctgggacgt cctataattt cttgggcagt gaacccatca tctctcgaaa ctcttcccac
      601 agtcccttga ggacggagta tgccatcccg tggctgaacg agcctagctt cgtctttgct
      661 gacgtgatcc agaaaagccc agatggtccg gagggtgaag atgacaaggt ctacttcttt
      721 tttacggagg tatccgtgga gtacgaattc gtcttcaagt tgatgatccc gcgagttgcc
      781 agggtgtgca agggcgacca gggcggcctg cggactttgc aaaaaaaatg gacgtcattc
      841 ctaaaggcca ggctgatctg ctccaagcca gacagtggcc tggtcttcaa catacttcag
      901 gatgtgtttg tgctgagggc cccgggcctc aaggagcctg tgttctatgc ggtcttcacc
      961 ccacagctga acaatgtggg tctgtcagcg gtgtgcgcct acacactggc cacggtggag
     1021 gcagtcttct cccgtggaaa gtacatgcag agtgccacag tggagcagtc tcacaccaag
     1081 tgggtgcgct acaatggccc agtgcccact ccccgacctg gagcgtgtat cgacagtgag
     1141 gcccgggcag ccaactacac cagctccttg aatctcccag acaaaacact gcagtttgta
     1201 aaagaccacc ctttgatgga tgactcagtg accccgatag acaacagacc caagctgatc
     1261 aaaaaagatg taaactacac ccagatagtg gtagacagga cccaggccct ggatgggact
     1321 ttctacgacg tcatgttcat cagcacagac cggggagctc tgcataaagc agtcatcctc
     1381 acaaaagagg tgcatgtcat cgaggagacc caactcttcc gggactctga accggtccta
     1441 actctgctgc tatcatcaaa gaaggggagg aagtttgtct atgcaggctc caactctgga
     1501 gtggtccaag cgcccctggc attctgcgaa aagcacggta gctgtgaaga ctgtgtgtta
     1561 gcacgggacc cctactgtgc ctggagccca gccatcaagg cctgtgttac cctgcaccag
     1621 gaagaggcct ccagcagggg ctggattcag gacatgagcg gtgacacatc ctcatgcctg
     1681 gataagagta aagaaagttt caaccagcat tttttcaagc acggcggcac agcagaactc
     1741 aaatgtttcc aaaagtccaa cctagcccgg gtggtatgga agttccagaa tggcgagttg
     1801 aaggccgcaa gtcccaagta cggctttgtg ggcaggaagc acctgctcat cttcaacctg
     1861 tcggacggag acagcggcgt gtaccagtgc ctgtcagagg aaagggtgag gaataaaacg
     1921 gtctcccagc tgctggccaa gcacgttctg gaagtgaaga tggtacctct gctggaatcc
     1981 aacatcaagg ttctgcccac atggtccacc ccggtgcagc caatggccct gattgtgctg
     2041 gggggcgtcg ccggcctcct gcttttcatt gggctaggca tcttcttctg tgtcaggtgc
     2101 cggcaccgaa ggcgccaagc agagcggatg tctcagatca agagactcct cagtgagaag
     2161 aagacctgcc agtgccctca ccggtttcag aagacatgta gccccattgg agcggccgac
     2221 tacaaggacg acgatgacaa gtga
//
