LOCUS       sequence-79488-e        8895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Artificial transcription factor with VP16 targeting c-Fos (TALE A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Senecal A, Munsky B, Proux F, Ly N, Braye FE, Zimmer C, Mueller F, 
            Darzacq X
  TITLE     Transcription Factors Modulate c-Fos Transcriptional Bursts.
  JOURNAL   Cell Rep. 2014 Jun 25. pii: S2211-1247(14)00447-1. doi: 
            10.1016/j.celrep.2014.05.053.
  PUBMED    24981864
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2014 Jun 25. pii: S2211-1247(14)00447-1. doi: 
            10.1016/j.celrep.2014.05.053."
COMMENT     Sequence Label: pAF1-VP16-SeqFosA
FEATURES             Location/Qualifiers
     source          1..8895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      917..926
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4220..4453
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(4489..4506)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4495..4719
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4765..5193
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4852..4871)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5062..5083
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5202..5222)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5207..5536
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5387..5522
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5449..5468
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5603..6397
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5657..5676)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6267..6286
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6571..6692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6608..6627)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6662..6681
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6741..6757)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6741..6757)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6754..6776)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6765..6781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6789..6795,6796..6813,6814..6819))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6834..6855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6972..6989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7143..7728)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7223..7242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7899..8690,8691..8759))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8522..8541
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8760..8864)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatggaccc cattcgtccg cgcaggccaa gtcctgcccg
      961 cgagcttctg cctggacccc aaccggatag ggttcagccg actgcagatc gtggggtgtc
     1021 tgcgcctgct ggcagccctc tggatggctt gcccgctcgg cggacggtgt cccggacccg
     1081 gctgccatct ccccctgcgc cctcacctgc gttctcggcg ggcagcttca gcgatctgct
     1141 ccgtccgttc gatccgtcgc ttcttgatac atcgcttctt gattcgatgc ctgccgtcgg
     1201 cacgccgcat acagcggctg ccccagcaga gtgggatgag gcgcaatcgg ctctgcgtgc
     1261 agccgatgac ccgccaccca ccgtgcgtgt cgctgtcact gccgcgcggc cgccgcgcgc
     1321 caagccggcc ccgcgacggc gtgctgcgca accctccgac gcttcgccgg ccgcgcaggt
     1381 ggatctacgc acgctcggct acagtcagca gcagcaagag aagatcaaac cgaaggtgcg
     1441 ttcgacagtg gcgcagcacc acgaggcact ggtgggccat gggtttacac acgcgcacat
     1501 cgttgcgctc agccaacacc cggcagcgtt agggaccgtc gctgtcacgt atcagcacat
     1561 aatcacggcg ttgccagagg cgacacacga agacatcgtt ggcgtcggca aacagtggtc
     1621 cggcgcacgc gccctggagg ccttgctcac ggatgcgggg gagttgagag gtccgccgtt
     1681 acagttggac acaggccaac ttgtgaagat tgcaaaacgt ggcggcgtga ccgcaatgga
     1741 ggcagtgcat gcatcgcgca atgcactgac gggtgccccc ctgaacctga ccccggacca
     1801 agtggtggct atcgccagcc acgatggcgg caagcaagcg ctcgaaacgg tgcagcggct
     1861 gttgccggtg ctgtgccagg accatggcct gactccggac caagtggtgg ctatcgccag
     1921 ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
     1981 ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacaatg gcggcaagca
     2041 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgactcc
     2101 ggaccaagtg gtggctatcg ccagccacga tggcggcaag caagcgctcg aaacggtgca
     2161 gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat
     2221 cgccagcaac attggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
     2281 gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca acggtggcgg
     2341 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
     2401 gaccccggac caagtggtgg ctatcgccag caacggtggc ggcaagcaag cgctcgaaac
     2461 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt
     2521 ggctatcgcc agcaacaatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc
     2581 ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacat
     2641 tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca
     2701 tggcctgacc ccggaccaag tggtggctat cgccagcaac attggcggca agcaagcgct
     2761 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca
     2821 agtggtggct atcgccagcc acgatggcgg caagcaagcg ctcgaaacgg tgcagcggct
     2881 gttgccggtg ctgtgccagg accatggcct gactccggac caagtggtgg ctatcgccag
     2941 ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
     3001 ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacattg gcggcaagca
     3061 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc
     3121 ggaccaagtg gtggctatcg ccagcaacaa tggcggcaag caagcgctcg aaacggtgca
     3181 gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat
     3241 cgccagcaac aatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
     3301 gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca acggtggcgg
     3361 caagcaagcg ctcgaaagca ttgtggccca gctgagccgg cctgatccgg cgttggccgc
     3421 gttgaccaac gaccacctcg tcgccttggc ctgcctcggc ggacgtcctg ccatggatgc
     3481 agtgaaaaag ggattgccgc acgcgccgga attgatcaga agagtcaatc gccgtattgg
     3541 cgaacgcacg tcccatcgcg ttgccgacta cgcgcaagtg gttcgcgtgc tggagttttt
     3601 ccagtgccac tcccacccag cgtacgcatt tgatgaggcc atgacgcagt tcgggatgag
     3661 caggaacggg ttggtacagc tctttcgcag agtgggcgtc accgaactcg aagcccgcgg
     3721 tggaacgctc cccccagcct cgcagcgttg ggaccgtatc ctccaggcat cagggatgaa
     3781 aagggccaaa ccgtccccta cttcagctca aacaccggat caggcgtctt tgcatgcatt
     3841 cgccgattcg ctggagcgtg accttgatgc gcccagccca atgcacgagg gagatcagac
     3901 gcgggcaagc agccgtaaac ggtcccgatc ggatcgtgct gtcaccggcc cctccgcaca
     3961 gcaggctgtc gaggtgcgcg ttcccgaaca gcgcgatgcg ctgcatttgc ccctcagctg
     4021 gagggtaaaa cgcccgcgta ccaggatctg gggcggcctc ccggatccac cgtacagccg
     4081 cgcgcgtacg aaaaacaatt acgggtctac catcgagggc ctgctcgatc tcccggacga
     4141 cgacgccccc gaagaggcgg ggctggcggc tccgcgcctg tcctttctcc ccgcgggaca
     4201 cacgcgcaga ctgtcgacgg cccccccgac cgatgtcagc ctgggggacg agctccactt
     4261 agacggcgag gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat
     4321 gttgggggac ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg
     4381 cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc ttggaattga
     4441 cgagtacggt gggtaggtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     4501 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4561 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4621 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4681 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4741 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4801 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4861 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4921 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4981 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5041 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5101 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5161 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     5221 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5281 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5341 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5401 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5461 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5521 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     5581 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     5641 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     5701 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     5761 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     5821 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     5881 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     5941 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     6001 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     6061 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     6121 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     6181 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     6241 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     6301 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     6361 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     6421 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     6481 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6541 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     6601 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6661 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     6721 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6781 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6841 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6901 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6961 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7021 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7081 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7141 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7201 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7261 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7321 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7381 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7441 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7501 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7561 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7621 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7681 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7741 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7801 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7861 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7921 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7981 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8041 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8101 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8161 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8221 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8281 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8341 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8401 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8461 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     8521 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8581 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8641 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8701 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8761 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8821 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8881 gtgccacctg acgtc
//
