LOCUS       sequence-79491-e        9609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Artificial transcription factor with VP16 targeting c-Fos (TALE C).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Senecal A, Munsky B, Proux F, Ly N, Braye FE, Zimmer C, Mueller F, 
            Darzacq X
  TITLE     Transcription Factors Modulate c-Fos Transcriptional Bursts.
  JOURNAL   Cell Rep. 2014 Jun 25. pii: S2211-1247(14)00447-1. doi: 
            10.1016/j.celrep.2014.05.053.
  PUBMED    24981864
REFERENCE   2  (bases 1 to 9609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2014 Jun 25. pii: S2211-1247(14)00447-1. doi: 
            10.1016/j.celrep.2014.05.053."
COMMENT     Sequence Label: pAF1-VP16-SeqFosC
FEATURES             Location/Qualifiers
     source          1..9609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      917..926
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4934..5167
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(5203..5220)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5209..5433
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5479..5907
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5566..5585)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5776..5797
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5916..5936)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5921..6250
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6101..6236
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6163..6182
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6317..7111
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6371..6390)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6981..7000
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7285..7406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7322..7341)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7376..7395
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7455..7471)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7455..7471)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7468..7490)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7479..7495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7503..7509,7510..7527,7528..7533))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7548..7569
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7686..7703)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7857..8442)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7937..7956)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8613..9404,9405..9473))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9236..9255
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9474..9578)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatggaccc cattcgtccg cgcaggccaa gtcctgcccg
      961 cgagcttctg cctggacccc aaccggatag ggttcagccg actgcagatc gtggggtgtc
     1021 tgcgcctgct ggcagccctc tggatggctt gcccgctcgg cggacggtgt cccggacccg
     1081 gctgccatct ccccctgcgc cctcacctgc gttctcggcg ggcagcttca gcgatctgct
     1141 ccgtccgttc gatccgtcgc ttcttgatac atcgcttctt gattcgatgc ctgccgtcgg
     1201 cacgccgcat acagcggctg ccccagcaga gtgggatgag gcgcaatcgg ctctgcgtgc
     1261 agccgatgac ccgccaccca ccgtgcgtgt cgctgtcact gccgcgcggc cgccgcgcgc
     1321 caagccggcc ccgcgacggc gtgctgcgca accctccgac gcttcgccgg ccgcgcaggt
     1381 ggatctacgc acgctcggct acagtcagca gcagcaagag aagatcaaac cgaaggtgcg
     1441 ttcgacagtg gcgcagcacc acgaggcact ggtgggccat gggtttacac acgcgcacat
     1501 cgttgcgctc agccaacacc cggcagcgtt agggaccgtc gctgtcacgt atcagcacat
     1561 aatcacggcg ttgccagagg cgacacacga agacatcgtt ggcgtcggca aacagtggtc
     1621 cggcgcacgc gccctggagg ccttgctcac ggatgcgggg gagttgagag gtccgccgtt
     1681 acagttggac acaggccaac ttgtgaagat tgcaaaacgt ggcggcgtga ccgcaatgga
     1741 ggcagtgcat gcatcgcgca atgcactgac gggtgccccc ctgaacctga ccccggacca
     1801 agtggtggct atcgccagcc acgatggcgg caagcaagcg ctcgaaacgg tgcagcggct
     1861 gttgccggtg ctgtgccagg accatggcct gactccggac caagtggtgg ctatcgccag
     1921 ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
     1981 ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacattg gcggcaagca
     2041 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgactcc
     2101 ggaccaagtg gtggctatcg ccagccacga tggcggcaag caagcgctcg aaacggtgca
     2161 gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat
     2221 cgccagcaac aatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
     2281 gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca acaatggcgg
     2341 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
     2401 gactccggac caagtggtgg ctatcgccag ccacgatggc ggcaagcaag cgctcgaaac
     2461 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgactccgg accaagtggt
     2521 ggctatcgcc agccacgatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc
     2581 ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg
     2641 tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca
     2701 tggcctgacc ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct
     2761 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca
     2821 agtggtggct atcgccagca acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct
     2881 gttgccggtg ctgtgccagg accatggcct gactccggac caagtggtgg ctatcgccag
     2941 ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
     3001 ggaccatggc ctgactccgg accaagtggt ggctatcgcc agccacgatg gcggcaagca
     3061 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgactcc
     3121 ggaccaagtg gtggctatcg ccagccacga tggcggcaag caagcgctcg aaacggtgca
     3181 gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat
     3241 cgccagcaac ggtggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
     3301 gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca acaatggcgg
     3361 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
     3421 gaccccggac caagtggtgg ctatcgccag caacggtggc ggcaagcaag cgctcgaaac
     3481 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt
     3541 ggctatcgcc agcaacattg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc
     3601 ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacaa
     3661 tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca
     3721 tggcctgact ccggaccaag tggtggctat cgccagccac gatggcggca agcaagcgct
     3781 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca
     3841 agtggtggct atcgccagcc acgatggcgg caagcaagcg ctcgaaacgg tgcagcggct
     3901 gttgccggtg ctgtgccagg accatggcct gactccggac caagtggtgg ctatcgccag
     3961 ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
     4021 ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacggtg gcggcaagca
     4081 agcgctcgaa agcattgtgg cccagctgag ccggcctgat ccggcgttgg ccgcgttgac
     4141 caacgaccac ctcgtcgcct tggcctgcct cggcggacgt cctgccatgg atgcagtgaa
     4201 aaagggattg ccgcacgcgc cggaattgat cagaagagtc aatcgccgta ttggcgaacg
     4261 cacgtcccat cgcgttgccg actacgcgca agtggttcgc gtgctggagt ttttccagtg
     4321 ccactcccac ccagcgtacg catttgatga ggccatgacg cagttcggga tgagcaggaa
     4381 cgggttggta cagctctttc gcagagtggg cgtcaccgaa ctcgaagccc gcggtggaac
     4441 gctcccccca gcctcgcagc gttgggaccg tatcctccag gcatcaggga tgaaaagggc
     4501 caaaccgtcc cctacttcag ctcaaacacc ggatcaggcg tctttgcatg cattcgccga
     4561 ttcgctggag cgtgaccttg atgcgcccag cccaatgcac gagggagatc agacgcgggc
     4621 aagcagccgt aaacggtccc gatcggatcg tgctgtcacc ggcccctccg cacagcaggc
     4681 tgtcgaggtg cgcgttcccg aacagcgcga tgcgctgcat ttgcccctca gctggagggt
     4741 aaaacgcccg cgtaccagga tctggggcgg cctcccggat ccaccgtaca gccgcgcgcg
     4801 tacgaaaaac aattacgggt ctaccatcga gggcctgctc gatctcccgg acgacgacgc
     4861 ccccgaagag gcggggctgg cggctccgcg cctgtccttt ctccccgcgg gacacacgcg
     4921 cagactgtcg acggcccccc cgaccgatgt cagcctgggg gacgagctcc acttagacgg
     4981 cgaggacgtg gcgatggcgc atgccgacgc gctagacgat ttcgatctgg acatgttggg
     5041 ggacggggat tccccgggtc cgggatttac cccccacgac tccgccccct acggcgctct
     5101 ggatatggcc gacttcgagt ttgagcagat gtttaccgat gcccttggaa ttgacgagta
     5161 cggtgggtag gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     5221 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     5281 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     5341 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     5401 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     5461 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5521 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5581 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5641 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5701 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5761 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5821 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5881 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     5941 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6001 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6061 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     6121 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6181 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6241 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     6301 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     6361 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     6421 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     6481 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     6541 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     6601 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     6661 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     6721 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     6781 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     6841 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     6901 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     6961 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     7021 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     7081 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     7141 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     7201 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     7261 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     7321 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7381 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     7441 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7501 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7561 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7621 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7681 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7741 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7801 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7861 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7921 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7981 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8041 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8101 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8161 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8221 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8281 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8341 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt
     8401 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8461 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8521 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8581 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8641 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8701 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8761 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8821 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8881 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8941 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9001 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9061 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9121 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9181 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9241 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9301 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9361 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9421 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9481 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9541 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9601 cctgacgtc
//
