LOCUS       sequence-79494-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express C-terminally triple HA tagged human PGAP3 in mammmalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Castillon GA, Michon L, Watanabe R
  TITLE     Apical sorting of lysoGPI-anchored proteins occurs independent of 
            association with detergent-resistant membranes but dependent on 
            their N-glycosylation.
  JOURNAL   Mol Biol Cell. 2013 Jun;24(12):2021-33. doi: 
            10.1091/mbc.E13-03-0160. Epub 2013 Apr 24.
  PUBMED    23615438
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-03-0160"; journalName: "Mol Biol Cell"; date: 
            "2013-06"; volume: "24"; issue: "12"; pages: "2021-33"
COMMENT     Sequence Label: pME-hygro-hPGAP3-3HA
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    260..394
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(284..303)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     338..357
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(488..514)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(521..547)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(554..580)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gaggaattaa
      241 ttcgagctcg gtacccgggg atccagacat gataagatac attgatgagt ttggacaaac
      301 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      361 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
      421 gtttcaggtt cagggggagg tgtgggaggt tttttctcta gactagtcta gcggccgctt
      481 aggcgccagc gtaatcaggc acgtcatagg ggtatccgcc agcgtaatca ggcacgtcat
      541 aggggtatcc gccagcgtaa tcaggcacgt cataggggta tccgccacgc gtgtccagct
      601 tgaacttgtc ctctgattcc ttcagcaggt acaggctgtc atcttccaga aagctgaaaa
      661 agaggacgtg gacagggatg gtgctgatgt gccagatggc atgggcatcc aggacccaga
      721 agagcggtgg gaagtcaagc agctcgagca gggacagccc ctgcagcagc aagaccacca
      781 ccacgcactt gcgcacgtga ggcagccgcc gctggttcca caggcacca
//
