LOCUS       sequence-79495-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express C-terminally triple HA tagged human PGAP3 in mammmalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Castillon GA, Michon L, Watanabe R
  TITLE     Apical sorting of lysoGPI-anchored proteins occurs independent of 
            association with detergent-resistant membranes but dependent on 
            their N-glycosylation.
  JOURNAL   Mol Biol Cell. 2013 Jun;24(12):2021-33. doi: 
            10.1091/mbc.E13-03-0160. Epub 2013 Apr 24.
  PUBMED    23615438
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-03-0160"; journalName: "Mol Biol Cell"; date: 
            "2013-06"; volume: "24"; issue: "12"; pages: "2021-33"
COMMENT     Sequence Label: pME-hygro-hPGAP3-3HA
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(132..461)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      146..281
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(200..219)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     446..466
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(629..650)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc
       61 gcggtgagtt caggcttttt catcacgtgc tgatcagatc cgaaaatgga tatacaagct
      121 cccgggagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat
      181 agctcagagg cagaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
      241 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
      301 actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
      361 ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
      421 gctggggagc ctggggactt tccacaccct aactgacaca cattccacag aattagcttg
      481 gtggcgaatg gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
      541 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
      601 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
      661 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
      721 accatcaccc taatcaagtt ttttggggtc gaggtgccgt a
//
