LOCUS       sequence-79508-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for 
            measurement of cotranslational protein degradation, use with 50572.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Turner GC, Varshavsky A
  TITLE     Detecting and measuring cotranslational protein degradation in vivo.
  JOURNAL   Science. 2000 Sep 22;289(5487):2117-20.
  PUBMED    11000112
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2000-09-22"; volume: "289(5487)"; pages: "2117-20"
COMMENT     Sequence Label: pDBD-R
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(768..794)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gccgagttaa cgccatcaaa aataattcgc gtctggcctt cctgtagcca gctttcatca
       61 acattaaatg tgagcgagta acaacccgtc ggattctccg tgggaacaaa cggcggattg
      121 accgtaatgg gataggtcac gttggtgtag atgggcgcat cgtaaccgtg catctgccag
      181 tttgagggga cgacgacagt atcggcctca ggaagatcgc actccagcca gctttccggc
      241 accgcttctg gtgccggaaa ccaggcaaag cgccattcgc cattcaggct gcgcaactgt
      301 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      361 gctgcaaggc gattaagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      421 gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag
      481 acgggcaaca gccaagctcc ggatccgtgt ctgccaccgc ggagccttag cacaagatgt
      541 aaggtggact ccttctgaat gttgtaatca gacagcgttc taccgtcttc tagctgccta
      601 ccggcaaaga tcaatctttg ttgatctgga gggatacctt ccttgtcttg aattttcgac
      661 ttaacgttgt cgatggtatc ggaagattca acttccaatg ttatggtttt accggtcaaa
      721 gtcttgacga aaatctgcat ggtcgaccca ctacccgcta aaaatgcagc gtaatctgga
      781 acatcgtatg ggtaggtacc gtctttcttc tcgtagactt caaacttata cttgatgcct
      841 tttttcctcc tgg
//
