LOCUS       sequence-79510-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of human alpha-synuclein in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  TITLE     MJFF plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET21a-alpha-synuclein
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             474..491
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(544..562)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      558..605
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(729..748)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata tacatatgga tgtgtttatg aaaggtctga
       61 gcaaagcgaa agaaggcgtg gtggctgcgg cggaaaaaac gaaacagggc gtggcggaag
      121 cggccggcaa aacgaaagaa ggtgttctgt atgtcggcag caaaaccaaa gaaggcgtgg
      181 ttcatggtgt ggccaccgtt gcagaaaaaa cgaaagaaca ggtcaccaac gtgggcggtg
      241 ctgtcgtgac cggtgttacg gctgtcgcgc aaaaaacggt ggaaggcgcg ggttctattg
      301 cggcggcaac cggtttcgtt aaaaaagatc agctgggtaa aaatgaagaa ggcgcgccgc
      361 aagaaggtat cctggaagac atgccggtgg atccggacaa cgaagcgtat gaaatgccgt
      421 cggaagaagg ctatcaagac tatgaaccgg aagcgtaagc ggccgcactc gagcaccacc
      481 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      541 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      601 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
      661 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      721 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      781 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      841 ccccaaaaa
//
