LOCUS       sequence-79511-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Koivisto L, Hakkinen L, Matsumoto K, McCulloch CA, Yamada KM, 
            Larjava H
  TITLE     Glycogen synthase kinase-3 regulates cytoskeleton and translocation 
            of Rac1 in long cellular extensions of human keratinocytes.
  JOURNAL   Exp Cell Res. 2004 Feb 1;293(1):68-80.
  PUBMED    14729058
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp Cell 
            Res."; date: "2004-02-1"; volume: "293(1)"; pages: "68-80"
COMMENT     Sequence Label: pRKVSV Rac
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..611)
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     regulatory      647..656
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(678..710)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             complement(711..743)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     regulatory      746..755
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(801..820)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(833..851)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(834..851)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 agctagcttc tgcaggtcga ctcctagaat tcttacaaca gcaggcattt tctcttcctc
       61 ttcttcacgg gaggcgggca gaggactgct cggatcgctt cgtcaaacac tgtcttgagg
      121 cctcgctgtg tgagcgccga gcactccagg tattttacag caccaatctc cttagccatg
      181 gctagaccct gcggataggt gatgggagtc agcttcttct ccttcagttt ctcgatcgtg
      241 tctttatcat ccctaagatc aagtttagtt cccactagga tgatgggagt gttgggacag
      301 tggtgccgca cctcaggata ccactttgca cggacatttt caaatgatgc aggactcaca
      361 agggaaaagc aaattaagaa cacatctgtt tgcggatagg atagggggcg taatctgtca
      421 taatcttctt gtccagctgt atcccataag cccagattca ccggttttcc atctaccata
      481 acattggcag aataattgtc aaagacagta gggatatatt ctccaggaaa tgcattggtt
      541 gtgtaactga tcagtaggca agttttacct acagctccgt ctcccaccac cacacacttg
      601 atggcctgca tggtggcgga tctctttagg tagaagtcgg tggatcccat ggtggcggcc
      661 tcgagaagct tggatccctt gcccagccgg ttcatctcga tgtcggtgta cttgcccagc
      721 cggttcatct cgatgtcggt gtatcccatg gtggcggctg aatcgataga accgaggtgc
      781 agttggacct gggagtggac acctgtggag agaaaggcaa agtggatgtt attctatagt
      841 gtcacctaaa tcgtatgtgt atgatacat
//
