LOCUS       sequence-79517-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon 
            configuration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pETM6-FAS
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             164..186
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    201..225
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(225..244)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(226..244)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(282..326)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 agagacggtt tcgttcgggg ccgcaatatg gcgttcacgg atacccgtac gggtaacgat
       61 ccattcatcc gaggtatcaa ccattttttc caggtcagca ttcgtgcgga cttgttccgg
      121 caggtaggag cccgtgccaa taatcttcgt gtacatatgt atatctcctt cttaaagtta
      181 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt
      241 attaatttcg cgggatcgag atcgatctcg atcctagtag acgagtccat gtgctggcgt
      301 tcaaatttcg cagcagcggt ttctttacca gactcgagta gaaggcagac gtatcctgga
      361 acagaccgac tttcaggtcg ctggcggtat agatcagacg accatcaacc agcacttcgc
      421 catccgccag gcccataatc agacgacggt taacaatgcg tttaaagtga atacggtagg
      481 tcactttttt cgctgtcggc agtacctgac cagtgaattt cacttcgcca acgcccagcg
      541 cgcggccttt accttcgccg cccagccagc cgaggtagaa ccctaccagc tgccacattg
      601 cgtccaggcc caggcatccc ggcataaccg gatcgccaat aaagtggcat ccgaagaacc
      661 acagatccgg attgatatcc agttctgctt caacataccc tttgtcgaag ttaccacccg
      721 tttcggtcat tttgaccaca cggtccatca tcagcatgtt cggtgctggc aattgcgggc
      781 ctttagcgcc aaacagttca ccgcgaccag aggcaagaag gtcttctttt gtataggatt
      841 cgcgtttatc taccatatgt atatctcct
//
