LOCUS       sequence-79519-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes fabD, accA, accB, accC, accD in pseudo-operon 
            configuration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pACM4-ACA
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             109..153
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     191..210
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        191..209
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    210..234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             249..271
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cagatcgaag cggacaaatc cggtaccgtg aaagcaattc tggtcgaaag tggacaaccg
       61 gtagaatttg acgagccgct ggtcgtcatc gagtaactcg agtctggtaa agaaaccgct
      121 gctgcgaaat ttgaacgcca gcacatggac tcgtctacta ggatcgagat cgatctcgat
      181 cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta
      241 gaaataattt tgtttaactt taagaaggag atatacatat gctggataaa attgttattg
      301 ccaaccgcgg cgagattgca ttgcgtattc ttcgtgcctg taaagaactg ggcatcaaga
      361 ctgtcgctgt gcactccagc gcggatcgcg atctaaaaca cgtattactg gcagatgaaa
      421 cggtctgtat tggccctgct ccgtcagtaa aaagttatct gaacatcccg gcaatcatca
      481 gcgccgctga aatcaccggc gcagtagcaa tccatccggg ttacggcttc ctctccgaga
      541 acgccaactt tgccgagcag gttgaacgct ccggctttat cttcattggc ccgaaagcag
      601 aaaccattcg cctgatgggc gacaaagtat ccgcaatcgc ggcgatgaaa aaagcgggcg
      661 tcccttgcgt accgggttct gacggcccgc tgggcgacga tatggataaa aaccgtgcca
      721 ttgctaaacg cattggttat ccggtgatta tcaaagcctc cggcggcggc ggcggtcgcg
      781 gtatgcgcgt agtgcgcggc gacgctgaac tggcacaatc catctccatg acccgtgcgg
      841 aagcgaaagc tgctttcagc aacgatatgg tttacat
//
