LOCUS       sequence-79521-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pCDM4-GLY
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             89..133
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     171..190
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        171..189
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    190..214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             229..251
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 actggtatcc tggtacgaca acgaaaccgg ttactccaac aaagttctgg acctgatcgc
       61 tcacatctcc aaataactcg agtctggtaa agaaaccgct gctgcgaaat ttgaacgcca
      121 gcacatggac tcgtctacta ggatcgagat cgatctcgat cccgcgaaat taatacgact
      181 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
      241 taagaaggag atatacataa tacataatgt cagaacgttt cccaaatgac gtggatccga
      301 tcgaaactcg cgactggctc caggcgatcg aatcggtcat ccgtgaagaa ggtgttgagc
      361 gtgctcagta tctgatcgac caactgcttg ctgaagcccg caaaggcggt gtaaacgtag
      421 ccgcaggcac aggtatcagc aactacatca acaccatccc cgttgaagaa caaccggagt
      481 atccgggtaa tctggaactg gaacgccgta ttcgttcagc tatccgctgg aacgccatca
      541 tgacggtgct gcgtgcgtcg aaaaaagacc tcgaactggg cggccatatg gcgtccttcc
      601 agtcttccgc aaccatttat gatgtgtgct ttaaccactt cttccgtgca cgcaacgagc
      661 aggatggcgg cgacctggtt tacttccagg gccacatctc cccgggcgtg tacgctcgtg
      721 ctttcctgga aggtcgtctg actcaggagc agctggataa cttccgtcag gaagttcacg
      781 gcaatggcct ctcttcctat ccgcacccga aactgatgcc ggaattctgg cagttcccga
      841 ccgtatctat gggtctgggg ccgattggtg ctatttac
//
