LOCUS       sequence-79533-e        8684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expresses red fluorescent protein, mKate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Davidsohn N, Beal J, Kiani S, Adler A, Yaman F, Li Y, Xie Z, Weiss R
  TITLE     Accurate Predictions of Genetic Circuit Behavior from Part 
            Characterization and Modular Composition.
  JOURNAL   ACS Synth Biol. 2014 Nov 17.
  PUBMED    25369267
REFERENCE   2  (bases 1 to 8684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Nov 17."
COMMENT     Sequence Label: pExp_CAG:mKate
FEATURES             Location/Qualifiers
     source          1..8684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        107..410
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        413..688
                     /label=chicken beta-actin promoter
     intron          689..1702
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1626..1648
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    1761..1785
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1790..1799
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1796..2509
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     protein_bind    complement(2511..2535)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2625..2644)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2690..2745
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2744..2763)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3106..3122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3130..3146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3154..3160,3161..3178,3179..3184))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3199..3220
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    3393..4229
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(4259..4279)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4259..4277)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4298..4314)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4322..4338
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4346..4352,4353..4370,4371..4376))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4391..4412
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4529..4546)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4700..5288)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4780..4799)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5459..6250,6251..6319))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6082..6101
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6320..6424)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6450..6905)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6587..6608)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6799..6818
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7032..7054
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7046..7063
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7047..7063
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7073..7092
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7073..7091
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7106..7909
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    7988..8013
                     /label=SA
                     /note="splice acceptor site"
ORIGIN
        1 caactttgta tagaaaagtt ggctccgaat ttctcgagtt aattaatagt tattaatagt
       61 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      121 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      181 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggactatt
      241 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      301 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      361 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ggtcgaggtg
      421 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
      481 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggcgcgcgcc
      541 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc
      601 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
      661 ctataaaaag cgaagcgcgc ggcgggcggg agtcgagtcg ctgcgttgcc ttcgccccgt
      721 gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
      781 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
      841 cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg
      901 tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
      961 cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     1021 cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga
     1081 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     1141 ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     1201 cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     1261 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     1321 aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     1381 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag
     1441 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg
     1501 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     1561 tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg gacggggcag
     1621 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     1681 tgccttcttc tttttcctac agctcctggg caacgtgctg gttgttgtgc tgtctcatca
     1741 ttttggcaaa gaattcgacc ccaagtttgt acaaaaaagc aggctgatcg ccaccatggt
     1801 gtctaagggc gaagagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
     1861 cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     1921 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
     1981 ggctaccagc ttcatgtacg gcagcaaaac cttcatcaac cacacccagg gcatccccga
     2041 cttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga
     2101 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
     2161 cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact
     2221 cggctgggag gcctccaccg agatgctgta ccccgctgac ggcggcctgg aaggcagaag
     2281 cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttga agaccacata
     2341 cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg tggacagaag
     2401 actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg aggtggctgt
     2461 ggccagatac tgcgacctcc ctagcaaact ggggcacaaa cttaattgat acccagcttt
     2521 cttgtacaaa gtggtacgcg tgaattcact cctcaggtgc aggctgccta tcagaaggtg
     2581 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc
     2641 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
     2701 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     2761 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     2821 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
     2881 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     2941 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     3001 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     3061 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
     3121 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     3181 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     3241 cgctttccag tcgggaaacc tgtcgtgcca gcggatcgac agtactaagc ttggtagata
     3301 agttgattta gccataaaat attgtttccg tgacccctta attaataggg ataacagggt
     3361 aatctcgagg tttaaacgtc gacgcggccg ctactaggga caggattggt gacagaaaag
     3421 ccccatcctt aggcctcctc cttcctagtc tcctgatatt gggtctaacc cccacctcct
     3481 gttaggcaga ttccttatct ggtgacacac ccccatttcc tggagccatc tctctccttg
     3541 ccagaacctc taaggtttgc ttacgatgga gccagagagg atcctgggag ggagagcttg
     3601 gcagggggtg ggagggaagg gggggatgcg tgacctgccc ggttctcagt ggccaccctg
     3661 cgctaccctc tcccagaacc tgagctgctc tgacgcggct gtctggtgcg tttcactgat
     3721 cctggtgctg cagcttcctt acacttccca agaggagaag cagtttggaa aaacaaaatc
     3781 agaataagtt ggtcctgagt tctaactttg gctcttcacc tttctagtcc ccaatttata
     3841 ttgttcctcc gtgcgtcagt tttacctgtg agataaggcc agtagccagc cccgtcctgg
     3901 cagggctgtg gtgaggaggg gggtgtccgt gtggaaaact ccctttgtga gaatggtgcg
     3961 tcctaggtgt tcaccaggtc gtggccgcct ctactccctt tctctttctc catccttctt
     4021 tccttaaaga gtccccagtg ctatctggga catattcctc cgcccagagc agggtcccgc
     4081 ttccctaagg ccctgctctg ggcttctggg tttgagtcct tggcaagccc aggagaggcg
     4141 ctcaggcttc cctgtccccc ttcctcgtcc accatctcat gcccctggct ctcctgcccc
     4201 ttccctacag gggttcctgg ctctgctctc caccgcggtg gagctccagc ttttgttccc
     4261 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     4321 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     4381 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     4441 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     4501 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4561 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4621 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4681 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4741 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4801 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4861 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4921 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4981 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5041 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5101 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5161 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5221 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5281 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5341 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5401 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5461 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5521 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5581 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5641 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5701 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5761 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5821 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5881 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5941 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6001 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6061 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     6121 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6181 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6241 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6301 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6361 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6421 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
     6481 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     6541 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     6601 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     6661 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     6721 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     6781 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     6841 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     6901 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     6961 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     7021 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     7081 ctcactatag ggcgaattgg gtacctgctt tctctgacca gcattctctc ccctgggcct
     7141 gtgccgcttt ctgtctgcag cttgtggcct gggtcacctc tacggctggc ccagatcctt
     7201 ccctgccgcc tccttcaggt tccgtcttcc tccactccct cttccccttg ctctctgctg
     7261 tgttgctgcc caaggatgct ctttccggag cacttccttc tcggcgctgc accacgtgat
     7321 gtcctctgag cggatcctcc ccgtgtctgg gtcctctccg ggcatctctc ctccctcacc
     7381 caaccccatg ccgtcttcac tcgctgggtt cccttttcct tctccttctg gggcctgtgc
     7441 catctctcgt ttcttaggat ggccttctcc gacggatgtc tcccttgcgt cccgcctccc
     7501 cttcttgtag gcctgcatca tcaccgtttt tctggacaac cccaaagtac cccgtctccc
     7561 tggctttagc cacctctcca tcctcttgct ttctttgcct ggacaccccg ttctcctgtg
     7621 gattcgggtc acctctcact cctttcattt gggcagctcc cctacccccc ttacctctct
     7681 agtctgtgct agctcttcca gccccctgtc atggcatctt ccaggggtcc gagagctcag
     7741 ctagtcttct tcctccaacc cgggccccta tgtccacttc aggacagcat gtttgctgcc
     7801 tccagggatc ctgtgtcccc gagctgggac caccttatat tcccagggcc ggttaatgtg
     7861 gctctggttc tgggtacttt tatctgtccc ctccacccca cagtggggca agcttattac
     7921 cctgttatcc ctaaccggtt gcttgcaaaa gcagtaattg gaaagcactc tcaaagaatc
     7981 caagcttctg acctcttctc ttcctcccac agggcctcga gacaattgca tcgatggtac
     8041 cagtgaaagt ctgcaatgaa ttcgagttgg cgcgcctgtc attctaaatc tctctttcag
     8101 cctaaagctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc
     8161 gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc
     8221 cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg
     8281 ccccctagcg ggggagggac gtaattacat ccctgggggc tttgggaggg ggctgtcccc
     8341 gtgagctccc aggcgcgcct gtcattctaa atctctcttt cagcctaaag ctttttcccc
     8401 gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat
     8461 cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg
     8521 gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg
     8581 gacgtaatta catccctggg ggctttgggg gggggctgtc cccgtgagct cccaggcgcg
     8641 ccaactcggt accgtatcga tgtcgacgtt aacgctagtg atat
//
