LOCUS       sequence-79542-e        9939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of TAL14 as input repressor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Davidsohn N, Beal J, Kiani S, Adler A, Yaman F, Li Y, Xie Z, Weiss R
  TITLE     Accurate Predictions of Genetic Circuit Behavior from Part 
            Characterization and Modular Composition.
  JOURNAL   ACS Synth Biol. 2014 Nov 17.
  PUBMED    25369267
REFERENCE   2  (bases 1 to 9939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Nov 17."
COMMENT     Sequence Label: pExp_TRE:TAL14
FEATURES             Location/Qualifiers
     source          1..9939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        36..350
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    44..62
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    115..133
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    151..169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    222..240
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    258..276
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     323..347
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    424..448
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3633..3653
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(3766..3790)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3880..3899)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3945..4000
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3999..4018)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4361..4377)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4385..4401
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4409..4415,4416..4433,4434..4439))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4454..4475
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    4648..5484
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(5514..5534)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5514..5532)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5553..5569)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5577..5593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5601..5607,5608..5625,5626..5631))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5646..5667
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5784..5801)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5955..6543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6035..6054)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6714..7505,7506..7574))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7337..7356
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7575..7679)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7705..8160)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7842..7863)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8054..8073
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8287..8309
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8301..8318
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8302..8318
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8328..8347
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8328..8346
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    8361..9164
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    9243..9268
                     /label=SA
                     /note="splice acceptor site"
ORIGIN
        1 caactttgta tagaaaagtt ggctccgaat ttctcgagtt tactccctat cagtgataga
       61 gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt ttactcccta
      121 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
      181 gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca gtgatagaga
      241 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac
      301 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagatttc
      361 gagctcggta cccggggatc ctctagtcag ctgacgcgtg ctagtggcgc gccgaattcg
      421 accccaagtt tgtacaaaaa agcaggctga ttaccggaga attccaattg accatgaccg
      481 gtttgtcgac cattcgtccg cgcaggccaa gtcctgcccg cgagcttctg cccggacccc
      541 aaccggatag ggttcagccg actgcagatc gtggggtgtc tgcgcctgct ggcagccctc
      601 tggatggctt gcccgctcgg cggacggtgt cccggacccg gctgccatct ccccctgcgc
      661 cctcacctgc gttctcggcg ggcagcttca gcgatctgct ccgtccgttc gatccgtcgc
      721 ttcttgatac atcgcttctt gattcgatgc ctgccgtcgg cacgccgcat acagcggctg
      781 ccccagcaga gtgggatgag gcgcaatcgg ctctgcgtgc agccgatgac ccgccaccca
      841 ccgtgcgtgt cgctgtcact gccgcgcggc cgccgcgcgc caagccggcc ccgcgacggc
      901 gtgctgcgca accctccgac gcttcgccgg ccgcgcaggt ggatctacgc acgctcggct
      961 acagtcagca gcagcaagag aagatcaaac cgaaggtgcg ttcgacagtg gcgcagcacc
     1021 acgaggcact ggtgggccat gggtttacac acgcgcacat cgttgcgctc agccaacacc
     1081 cggcagcgtt agggaccgtc gctgtcacgt atcagcacat aatcacggcg ttgccagagg
     1141 cgacacacga agacatcgtt ggcgtcggca aacagtggtc cggcgcacgc gccctggagg
     1201 ccttgctcac ggatgcgggg gagttgagag gtccgccgtt acagttggac acaggccaac
     1261 ttgtgaagat tgcaaaacgt ggcggcgtga ccgcaatgga ggcagtgcat gcatcgcgca
     1321 atgcactgac gggtgccccc ctgaacctga ccccggacca agtggtggct atcgccagca
     1381 acattggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg
     1441 accatggcct gactccggac caagtggtgg ctatcgccag ccacgatggc ggcaagcaag
     1501 cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgactccgg
     1561 accaagtggt ggctatcgcc agccacgatg gcggcaagca agcgctcgaa acggtgcagc
     1621 ggctgttgcc ggtgctgtgc caggaccatg gcctgactcc ggaccaagtg gtggctatcg
     1681 ccagccacga tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
     1741 gccaggacca tggcctgact ccggaccaag tggtggctat cgccagccac gatggcggca
     1801 agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga
     1861 ccccggacca agtggtggct atcgccagca acggtggcgg caagcaagcg ctcgaaacgg
     1921 tgcagcggct gttgccggtg ctgtgccagg accatggcct gactccggac caagtggtgg
     1981 ctatcgccag ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
     2041 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacggtg
     2101 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
     2161 gcctgactcc ggaccaagtg gtggctatcg ccagccacga tggcggcaag caagcgctcg
     2221 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgact ccggaccaag
     2281 tggtggctat cgccagccac gatggcggca agcaagcgct cgaaacggtg cagcggctgt
     2341 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca
     2401 acaatggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg
     2461 accatggcct gactccggac caagtggtgg ctatcgccag ccacgatggc ggcaagcaag
     2521 cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg
     2581 accaagtggt ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa acggtgcagc
     2641 ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg
     2701 ccagcaacgg tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
     2761 gccaggacca tggcctgact ccggaccaag tggtggctat cgccagccac gatggcggca
     2821 agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga
     2881 ccccggacca agtggtggct atcgccagca acggtggcgg caagcaagcg ctcgaaagca
     2941 ttgtggccca gctgagccgg cctgatccgg cgttggccgc gttgaccaac gaccacctcg
     3001 tcgccttggc ctgcctcggc ggacgtcctg ccatggatgc agtgaaaaag ggattgccgc
     3061 acgcgccgga attgatcaga agagtcaatc gccgtattgg cgaacgcacg tcccatcgcg
     3121 ttgccgacta cgcgcaagtg gttcgcgtgc tggagttttt ccagtgccac tcccacccag
     3181 cgtacgcatt tgatgaggcc atgacgcagt tcgggatgag caggaacggg ttggtacagc
     3241 tctttcgcag agtgggcgtc accgaactcg aagcccgcgg tggaacgctc cccccagcct
     3301 cgcagcgttg ggaccgtatc ctccaggcat cagggatgaa aagggccaaa ccgtccccta
     3361 cttcagctca aacaccggat caggcgtctt tgcatgcatt cgccgattcg ctggagcgtg
     3421 accttgatgc gcccagccca atgcacgagg gagatcagac gcgggcaagc agccgtaaac
     3481 ggtcccgatc ggatcgtgct gtcaccggcc cctccgcaca gcaggctgtc gaggtgcgcg
     3541 ttcccgaaca gcgcgatgcg ctgcatttgc ccctcagctg gagggtaaaa cgcccgcgta
     3601 ccaggatctg gggcggcctc ccggatccca gccccaagaa gaagagaaag gtggaggcca
     3661 gcggtggcgg ctcaaagctt ggtggcggct caactagtta agggcccggc gcgcctaagg
     3721 tacccccggg taactgaaaa tccaccggat ctagataact gatctaccca gctttcttgt
     3781 acaaagtggt acgcgtgaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
     3841 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     3901 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     3961 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     4021 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     4081 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     4141 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     4201 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     4261 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     4321 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     4381 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     4441 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     4501 tccagtcggg aaacctgtcg tgccagcgga tcgacagtac taagcttggt ctaataccca
     4561 atctctcgtc ttatccagat gttttatacg ccttaattaa tagggataac agggtaatct
     4621 cgaggtttaa acgtcgacgc ggccgctact agggacagga ttggtgacag aaaagcccca
     4681 tccttaggcc tcctccttcc tagtctcctg atattgggtc taacccccac ctcctgttag
     4741 gcagattcct tatctggtga cacaccccca tttcctggag ccatctctct ccttgccaga
     4801 acctctaagg tttgcttacg atggagccag agaggatcct gggagggaga gcttggcagg
     4861 gggtgggagg gaaggggggg atgcgtgacc tgcccggttc tcagtggcca ccctgcgcta
     4921 ccctctccca gaacctgagc tgctctgacg cggctgtctg gtgcgtttca ctgatcctgg
     4981 tgctgcagct tccttacact tcccaagagg agaagcagtt tggaaaaaca aaatcagaat
     5041 aagttggtcc tgagttctaa ctttggctct tcacctttct agtccccaat ttatattgtt
     5101 cctccgtgcg tcagttttac ctgtgagata aggccagtag ccagccccgt cctggcaggg
     5161 ctgtggtgag gaggggggtg tccgtgtgga aaactccctt tgtgagaatg gtgcgtccta
     5221 ggtgttcacc aggtcgtggc cgcctctact ccctttctct ttctccatcc ttctttcctt
     5281 aaagagtccc cagtgctatc tgggacatat tcctccgccc agagcagggt cccgcttccc
     5341 taaggccctg ctctgggctt ctgggtttga gtccttggca agcccaggag aggcgctcag
     5401 gcttccctgt cccccttcct cgtccaccat ctcatgcccc tggctctcct gccccttccc
     5461 tacaggggtt cctggctctg ctctccaccg cggtggagct ccagcttttg ttccctttag
     5521 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     5581 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5641 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5701 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5761 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5821 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5881 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5941 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6001 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6061 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6121 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6181 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6241 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6301 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6361 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6421 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6481 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6541 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6601 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6661 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6721 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6781 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6841 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6901 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6961 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7021 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7081 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7141 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7201 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7261 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7321 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7381 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7441 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7501 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7561 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7621 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7681 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     7741 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     7801 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     7861 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     7921 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     7981 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     8041 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     8101 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     8161 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     8221 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     8281 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
     8341 tatagggcga attgggtacc tgctttctct gaccagcatt ctctcccctg ggcctgtgcc
     8401 gctttctgtc tgcagcttgt ggcctgggtc acctctacgg ctggcccaga tccttccctg
     8461 ccgcctcctt caggttccgt cttcctccac tccctcttcc ccttgctctc tgctgtgttg
     8521 ctgcccaagg atgctctttc cggagcactt ccttctcggc gctgcaccac gtgatgtcct
     8581 ctgagcggat cctccccgtg tctgggtcct ctccgggcat ctctcctccc tcacccaacc
     8641 ccatgccgtc ttcactcgct gggttccctt ttccttctcc ttctggggcc tgtgccatct
     8701 ctcgtttctt aggatggcct tctccgacgg atgtctccct tgcgtcccgc ctccccttct
     8761 tgtaggcctg catcatcacc gtttttctgg acaaccccaa agtaccccgt ctccctggct
     8821 ttagccacct ctccatcctc ttgctttctt tgcctggaca ccccgttctc ctgtggattc
     8881 gggtcacctc tcactccttt catttgggca gctcccctac cccccttacc tctctagtct
     8941 gtgctagctc ttccagcccc ctgtcatggc atcttccagg ggtccgagag ctcagctagt
     9001 cttcttcctc caacccgggc ccctatgtcc acttcaggac agcatgtttg ctgcctccag
     9061 ggatcctgtg tccccgagct gggaccacct tatattccca gggccggtta atgtggctct
     9121 ggttctgggt acttttatct gtcccctcca ccccacagtg gggcaagctt attaccctgt
     9181 tatccctaac cggtgcgttt ttatgcttgt agtattgtat aatgttttta agatccaagc
     9241 ttctgacctc ttctcttcct cccacagggc ctcgagacaa ttgcatcgat ggtaccagtg
     9301 aaagtctgca atgaattcga gttggcgcgc ctgtcattct aaatctctct ttcagcctaa
     9361 agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca
     9421 gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct
     9481 cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc
     9541 tagcggggga gggacgtaat tacatccctg ggggctttgg gagggggctg tccccgtgag
     9601 ctcccaggcg cgcctgtcat tctaaatctc tctttcagcc taaagctttt tccccgtatc
     9661 cccccaggtg tctgcaggct caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt
     9721 gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg gctcggggat gcggggggag
     9781 cgccggaccg gagcggagcc ccgggcggct cgctgctgcc ccctagcggg ggagggacgt
     9841 aattacatcc ctgggggctt tggggggggg ctgtccccgt gagctcccag gcgcgccaac
     9901 tcggtaccgt atcgatgtcg acgttaacgc tagtgatat
//
