LOCUS       sequence-79554-e        2685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  P2A peptide from porcine teschovirus-1 flanked by restriction sites 
            in shuttle vector for easy cloning of multiple separate proteins to 
            be expressed from single message.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Daniels RW, Rossano AJ, Macleod GT, Ganetzky B
  TITLE     Expression of multiple transgenes from a single construct using 
            viral 2A peptides in Drosophila.
  JOURNAL   PLoS One. 2014 Jun 19;9(6):e100637. doi: 
            10.1371/journal.pone.0100637. eCollection 2014.
  PUBMED    24945148
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-06-19"; volume: "9(6):e100637. doi"; pages: " 
            10.1371/journal.pone.0100637. eCollection 2014"
COMMENT     Sequence Label: pC5Kan-P2A
FEATURES             Location/Qualifiers
     source          1..2685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             281..337
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(561..1355)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(672..691)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1282..1301
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(1822..1843)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1979..1996)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2150..2685,1..53))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2230..2249)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
       61 ctttgatctt ttctactgaa cggtgatccc caccggaatt gcggcctcag cagattaatc
      121 tggccggcct acttaattaa gtggaattca aaggtacccg cccgggatca gatccgcggc
      181 cgcataggcc actagtggat ctggatcctt cttaagaaga gctctaaagc tttatctaga
      241 gtcgacctcg aacgttaact cgaggcctat aagatccggc gccaccaact tctccctgct
      301 gaagcaggcc ggcgacgtgg aggagaaccc cggccccgcc cccagatctc ttgcatacta
      361 cctgcaggat accggtataa cgcgtatagc tagctttcct aggtctgcga tcgcaaaggc
      421 gcgccatagt ttaaacaagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
      481 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat
      541 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
      601 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
      661 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc
      721 agacggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
      781 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatacgcgc cttgagcctg
      841 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
      901 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
      961 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     1021 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     1081 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     1141 gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg
     1201 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     1261 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagccgcc
     1321 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     1381 tgatcagacc ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt
     1441 actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt
     1501 gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc
     1561 tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg
     1621 ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg
     1681 cgccctgagt gcttgcggca gcgtgaagct aattcatggt taaatttttg ttaaatcagc
     1741 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagccc
     1801 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     1861 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gccggatcag cttatgcggt
     1921 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     1981 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     2041 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2101 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctgacgttt ttccataggc
     2161 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2221 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2281 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2341 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2401 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2461 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2521 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2581 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2641 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcg
//
