LOCUS       sequence-79555-e          60 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  P2A peptide from porcine teschovirus-1 flanked by restriction sites 
            in shuttle vector for easy cloning of multiple separate proteins to 
            be expressed from single message.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 60)
  AUTHORS   Daniels RW, Rossano AJ, Macleod GT, Ganetzky B
  TITLE     Expression of multiple transgenes from a single construct using 
            viral 2A peptides in Drosophila.
  JOURNAL   PLoS One. 2014 Jun 19;9(6):e100637. doi: 
            10.1371/journal.pone.0100637. eCollection 2014.
  PUBMED    24945148
REFERENCE   2  (bases 1 to 60)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-06-19"; volume: "9(6):e100637. doi"; pages: " 
            10.1371/journal.pone.0100637. eCollection 2014"
COMMENT     Sequence Label: pC5Kan-P2A
FEATURES             Location/Qualifiers
     source          1..60
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..60
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ggcgccacca acttctccct gctgaagcag gccggcgacg tggaggagaa ccccggcccc
//
