LOCUS       sequence-79558-e         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses constituvely active cofilin fused to mRFP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Garvalov BK, Flynn KC, Neukirchen D, Meyn L, Teusch N, Wu X, 
            Brakebusch C, Bamburg JR, Bradke F
  TITLE     Cdc42 regulates cofilin during the establishment of neuronal 
            polarity.
  JOURNAL   J Neurosci. 2007 Nov 28;27(48):13117-29.
  PUBMED    18045906
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2007-11-28"; volume: "27(48)"; pages: "13117-29"
COMMENT     Sequence Label: pmRFP-N1 human cofilin S3A
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(170..193)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(368..387)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     637..655
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
       61 ctacaaatgt ggtatggctg attatgatct agagtcgcgg ccaattctta ggcgccggtg
      121 gagtggcggc cctcggcgcg ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag
      181 gtgatgtcca gcttgatgtc ggtcttgtag gcgccgggca gctgcacggg cttcttggcc
      241 atgtaggtgg tcttgacctc ggcgtcgtag tggccgccgt ccttcagctt cagcctcatc
      301 ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc gctcggtgga ggcctcccag
      361 cccatggtct tcttctgcat tacggggccg tcggagggga agttggtgcc gcgcagcttc
      421 accttgtaga tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg
      481 ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcaag
      541 tagtcgggga tgtcggcggg gtgcttcacg taggccttgg agccgtactg gaactgaggg
      601 gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg tcaccttcag cttggcggtc
      661 tgggtgccct cgta
//
