LOCUS       sequence-79559-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses psuedo-phosporylated, non-activatable cofilin fused to 
            mRFP in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Garvalov BK, Flynn KC, Neukirchen D, Meyn L, Teusch N, Wu X, 
            Brakebusch C, Bamburg JR, Bradke F
  TITLE     Cdc42 regulates cofilin during the establishment of neuronal 
            polarity.
  JOURNAL   J Neurosci. 2007 Nov 28;27(48):13117-29.
  PUBMED    18045906
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2007-11-28"; volume: "27(48)"; pages: "13117-29"
COMMENT     Sequence Label: pmRFP-N1 human cofilin S3E
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(101..778)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPLAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(163..186)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(361..380)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     630..648
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
       61 tgtggtatgg ctgattatga tctagagtcg cggccaattc ttaggcgccg gtggagtggc
      121 ggccctcggc gcgctcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt
      181 ccagcttgat gtcggtcttg taggcgccgg gcagctgcac gggcttcttg gccatgtagg
      241 tggtcttgac ctcggcgtcg tagtggccgc cgtccttcag cttcagcctc atcttgatct
      301 cgcccttcag ggcgccgtcc tcggggtaca tccgctcggt ggaggcctcc cagcccatgg
      361 tcttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt
      421 agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct
      481 cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg
      541 ggatgtcggc ggggtgcttc acgtaggcct tggagccgta ctggaactga ggggacagga
      601 tgtcccaggc gaggggcagg gggccgccct tggtcacctt cagcttggcg gtctgggtgc
      661 cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc
      721 cctccatgcg caccttgaag cgcatgaact ccttgatgac gtcctcggag gaggccatcg
      781 gatcccggga taacaaaggc ttgccctcca
//
