LOCUS       sequence-79563-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning E.coli expression vector with araBAD promoter and 
            C-terminal 3C protease cleavage site and 10x His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pBXC3H
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(40..69)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(80..109)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(110..133)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             160..180
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             184..204
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    223..347
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(372..377,378..395,396..402)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(522..541)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aactcaatga tgatgatgat gatggtcgac ggcgctattc agatcctctt ctgagatgag
       61 tttttgttct agattattaa tgatgatgat gatggtgatg atgatggtgt ggaccttgaa
      121 acaaaacttc taatgcagaa gagctgaact agtggatccc caaaaaagaa gagaaaggta
      181 gatccaaaaa agaagagaaa ggtagctagc gaattcgata tcacaagttt gtacaaaaaa
      241 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
      301 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcattag
      361 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
      421 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
      481 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
      541 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
      601 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
      661 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
      721 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
      781 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
      841 aaaacctgg
//
