LOCUS       sequence-79565-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning E.coli expression vector with araBAD promoter and 
            C-terminal 3C protease cleavage site, GFP and 10x His tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pBXC3GH
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(40..69)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(85..114)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(121..831)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(161..181)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     776..804
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(838..861)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 aactcaatga tgatgatgat gatggtcgac ggcgctattc agatcctctt ctgagatgag
       61 tttttgttct agatggtcga cttaatgatg gtgatgatga tggtgatgat gatgactagt
      121 tttgtagagc tcatccatgc catgtgtaat cccagcagca gttacaaact caagaaggac
      181 catgtggtca cgcttttcgt tgggatcttt cgaaagggca gattgtgtcg acaggtaatg
      241 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
      301 tagttgaacg gaaccatctt caatgttgtg gcgaattttg aagttagctt tgattccatt
      361 cttttgtttg tctgccgtga tgtatacatt gtgtgagtta tagttgtact ctagtttgtg
      421 tccgagaatg tttccatctt ctttaaaatc aatacccttt aactcgatac gattaacaag
      481 ggtatcacct tcaaacttga cttcagcacg cgtcttgtag ttcccgtcat ctttgaaaga
      541 tatagtgcgt tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgccgttt
      601 catgtgatcc ggataacggg aaaagcattg aacaccatag gtcagagtag tgacaagtgt
      661 tggccaagga acaggtagtt ttccagtagt gcaaataaat ttaagggtga gttttccgta
      721 tgtagcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc
      781 taattcaaca agaattggga caactccagt gaaaagttct tctcctttgc tgaattgtgg
      841 accttgaaac aaaacttcta atgca
//
