LOCUS       sequence-79567-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning E.coli expression vector with araBAD promoter and 
            C-terminal Avi-tag, 3C protease site and 10x His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pBXCA3H
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(40..69)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(80..109)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(110..133)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(134..178)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             205..225
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             229..249
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    268..392
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(417..422,423..440,441..447)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(567..586)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aactcaatga tgatgatgat gatggtcgac ggcgctattc agatcctctt ctgagatgag
       61 tttttgttct agattattaa tgatgatgat gatggtgatg atgatggtgt ggaccttgaa
      121 acaaaacttc taattcgtgc cattcgattt tctgagcttc gaagatatcg ttcagacctg
      181 cagaagagca aaactagtgg atccccaaaa aagaagagaa aggtagatcc aaaaaagaag
      241 agaaaggtag ctagcgaatt cgatatcaca agtttgtaca aaaaagctga acgagaaacg
      301 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      361 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta
      421 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt
      481 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
      541 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
      601 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
      661 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
      721 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
      781 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
      841 ggcagtttct acacatatat tcgcaagatg tgg
//
