LOCUS       sequence-79571-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Lactococcus lactis expression vector (VBEx) with nisin 
            promoter and C-terminal 3C protease cleavage site, GFP and 10x His 
            tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pREXC3GH
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(4..123)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(142..162)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(166..186)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             213..236
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(270..298)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
       61 atttaatata ttgatattta tatcatttta cgtttctcgt tcagcttttt tgtacaaact
      121 tgtgatatcg aattcgctag ctacctttct cttctttttt ggatctacct ttctcttctt
      181 ttttggggat ccactagttc agctcttctg cattagaagt tttgtttcaa ggtccacaat
      241 tcagcaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
      301 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgct acatacggaa
      361 aactcaccct taaatttatt tgcactactg gaaaactacc tgttccttgg ccaacacttg
      421 tcactactct gacctatggt gttcaatgct tttcccgtta tccggatcac atgaaacggc
      481 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaacgcact atatctttca
      541 aagatgacgg gaactacaag acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta
      601 atcgtatcga gttaaagggt attgatttta aagaagatgg aaacattctc ggacacaaac
      661 tagagtacaa ctataactca cacaatgtat acatcacggc agacaaacaa aagaatggaa
      721 tcaaagctaa cttcaaaatt cgccacaaca ttgaagatgg ttccgttcaa ctagcagacc
      781 attatcaaca aaatactcca attgg
//
