LOCUS       sequence-79572-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Lactococcus lactis expression vector (VBEx) with nisin 
            promoter and C-terminal 3C protease cleavage site and 10x His tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pREXC3H
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(4..123)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(142..162)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(166..186)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             213..236
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             237..266
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     terminator      722..749
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        768..859
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
       61 atttaatata ttgatattta tatcatttta cgtttctcgt tcagcttttt tgtacaaact
      121 tgtgatatcg aattcgctag ctacctttct cttctttttt ggatctacct ttctcttctt
      181 ttttggggat ccactagttc agctcttctg cattagaagt tttgtttcaa ggtccacacc
      241 atcatcatca ccatcatcat catcattaat aatctagaga gctcaagctt tctttgaacc
      301 aaaattagaa aaccaaggct tgaaacgttc aattgaaatg gcaattaaac aaattacagc
      361 acgtgttgct ttgattgata gccaaaaagc agcagttgat aaagcaatta ctgatattgc
      421 tgaaaaattg taatttataa ataaaaatca ccttttagag gtggtttttt tatttataaa
      481 ttattcgttt gatttcgctt tcgatagaac aatcaaagcg agaataagga agataaatcc
      541 cataagggcg ggagcagaat gtccgagact aatgtaaatt tgtccaccaa ttaaaggacc
      601 gataacgcga gcttctcgag atggccactg aggccgggag cggatttgaa cgttgcgaag
      661 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag
      721 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt
      781 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      841 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccc
//
