LOCUS       sequence-79574-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Saccharomyces cerevisiae expression vector with GAL1 
            promoter and N-terminal 10x His tag and 3C protease cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pYEXNH3
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..32
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13..31
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             61..90
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             91..114
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(163..468)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(265..284)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tactagcagc tgtaatacga ctcactatag ggaatattaa gcttggtacc attaaccatg
       61 caccatcatc atcaccatca tcatcatcat ttagaagttt tgtttcaagg tccaagtaga
      121 agagcgagct gcagactggc tgtgtataag ggagcctgac atttatattc cccagaacat
      181 caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc
      241 gataacggag accggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc
      301 gatatgcacc accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc
      361 cagggggatc accatccgtc gcccgggcgt gtcaataata tcactctgta catccacaaa
      421 cagacgataa cggctctctc ttttataggt gtaaacctta aactgcattt caccagcccc
      481 tgttctcgtc agcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt
      541 cctgattttc cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg
      601 tgcttaccag accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct
      661 gtcaactgtc actgtaatac gctgcttcat agcatacctc tttttgacat acttcgggta
      721 tacatatcag tatatattct tataccgcaa aaatcagcgc gcaaatacgc atactgttat
      781 ctggctttta gtaagccgga tccacgcgtt tacgccccgc cctgccactc atcgcagtac
      841 tgttgtaatt cattaagcat tctgccgaca t
//
