LOCUS       sequence-79575-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Saccharomyces cerevisiae expression vector with GAL1 
            promoter and N-terminal 10x His tag, GFP and 3C protease cleavage 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pYEXNHG3
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..36
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        17..35
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             58..87
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             88..798
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             805..828
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ggactactag cagctgtaat acgactcact atagggaata ttaagcttgg taccatgcac
       61 catcatcatc accatcatca tcatcattct aaaggagaag aattattcac tggtgttgtc
      121 ccaattttgg ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa
      181 ggtgaaggtg atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa
      241 ttgccagttc catggccaac cttagtcact actttcggtt atggtgttca atgttttgcg
      301 agatacccag atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat
      361 gttcaagaaa gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc
      421 aagtttgaag gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa
      481 gatggtaaca ttttaggtca caaattggaa tacaactata actctcacaa tgtttacatc
      541 atggctgaca aacaaaagaa tggtatcaaa gttaacttca aaattagaca caacattgaa
      601 gatggttctg ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca
      661 gtcttgttac cagacaacca ttacttatcc actcaatctg ccttatccaa agatccaaac
      721 gaaaagagag accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt
      781 atggatgaat tgtacaaaac tagtttagaa gttttgtttc aaggtccaag tagaa
//
