LOCUS       sequence-79578-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTAL plasmid compatible with the Golden Gate TALEN cloning system, 
            for cloning of TALENs and expression in mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTALEN(Nd154C63) MND
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..450)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     300..322
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     314..331
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     315..331
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     341..360
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        341..359
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(407..427)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(407..425)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(446..462)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(446..462)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(459..481)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    470..486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(494..500,501..518,519..524))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    539..560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gaggccttgc tcacggatgc gggggagttg agaggtccgc cgttacagtt ggacacaggc
       61 caacttgtga agattgcaaa acgtggcggc gtgaccgcaa tggaggcagt gcatgcatcg
      121 cgcaatgcac tgacgggtgc ccccctggag acgggcgccg ctacagggcg cgtcccattc
      181 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      241 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      301 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
      361 cgaattgggt accgggcccc ccctcgaggt cctccagctt ttgttccctt tagtgagggt
      421 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      481 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      541 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccac cggtcgtctc
      601 caacgaccac ctcgtcgcct tggcctgcct cggcggacgt cctgccatgg atgcagtgaa
      661 aaagggattg ccgcacgcgc cggaattgat cagaagagtc aatcgccgta ttggcgaacg
      721 cacgtcccat cgcgttgcga tatctagatc ccagctagtg aaatctgaat tggaagagaa
      781 gaaatctgaa cttagacata aattgaaata tgtgccacat gaatatattg aattgattga
      841 aatcgcaaga aattcaactc aggatagaat ccttgaaatg aaggtgatgg agttctttat
      901 gaaggtttat ggttatcgtg gtaaacattt gggtggatca aggaaaccag acggagcaat
      961 ttatactgtc ggatctccta ttgattacgg
//
