LOCUS       sequence-79580-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTAL plasmid compatible with the Golden Gate TALEN cloning system, 
            for cloning of TALENs and expression in mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTALEN(Nd154C63) Ef1a
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..807
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MASELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     CDS             811..864
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             871..897
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             901..921
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
       61 ttttcgcaac gggtttgccg ccagaacaca ggatctgccg ccaccatggc tagcgagctg
      121 attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc
      181 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
      241 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac
      301 ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc
      361 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
      421 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
      481 aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc
      541 gagacactgt accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc
      601 gtgggcggga gccatctgat cgcaaacatc aagaccacat atagatccaa gaaacccgct
      661 aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag
      721 gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc
      781 cctagcaaac tggggcacaa gcttaattca gagggcagag gaagtcttct aacatgcggt
      841 gacgtggagg agaatccggg cccctgcagg tatccatatg atgtcccaga ttatgcgcca
      901 cctaagaaga aacgcaaagt cgtggatcta cgcacgctcg gctacagtca gcagcagcaa
      961 gagaagatca aaccgaaggt gcgttcgaca gtggc
//
