LOCUS       sequence-79583-e         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRipTAL plasmid for cloning of megaTALs or other TAL-fusions and 
            expression in mammalian cells. RVDs are cloned between Age-Xho sites
            and meganuclease/fusion is cloned between Xba-Sal sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCVL RipTAL(Nd154C63) SFFV Age-Xho/Xba-Sal lin...
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(875..895)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(899..925)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(932..985)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 caatctttca caaattttgt aatccagagg ttgattaaca ggaacagagc gtaaataacg
       61 ggaaagtttc ttaacatgtt tgtcttgtgg caatacacct gaactagtaa ttacatatcc
      121 ctaaaaatgt aaatgattgc cccaccattt tgttctatta acatttaaat gtatacccaa
      181 atcaagaaaa acagaacaaa tatgggtata aatggcggta agatgctcgg tcgactgaga
      241 tcagatcact acgtcatcta gatatcgcaa cgcgatggga cgtgcgttcg ccaatacggc
      301 gattgactct tctgatcaat tccggcgcgt gcggcaatcc ctttttcact gcatccatgg
      361 caggacgtcc gccgaggcag gccaaggcga cgaggtggtc gttggtcaac gcggccaacg
      421 ccggatcagg ccggctcagc tgggccacaa tgctctcgag gtcagtcagc tagcatgcac
      481 cggtaagctt ccttcgcagc ctatacaggg cttggattgc tggccgctct ccgtactggg
      541 ccactgtggc gatctgggag gcggacagcc gcagcggggc ggcagtaagt gcggtagcca
      601 caggcagcag ggcttggagc gccagatctc cgctccgctg ccgggcgata tccactatgt
      661 gagcgcgagt cagttctggc agggcggcgg cgagctcggg gtagttccga gcgaccacgc
      721 gcaaactctg tctccgccga gaaattctgc agatatcagc gtgagtaaaa ccatgtccga
      781 ccaaggcctc atgatgctgg gcaaccgtgc ttcgcacttt aggcttaatt ttttcctgct
      841 gctgctgtga atagccgagg gttctgaggt ctacgacttt gcgtttcttc ttaggtggcg
      901 cataatctgg gacatcatat ggatacctgc aggggcccgg attctcctcc acgtcaccgc
      961 atgttagaag acttcctctg ccctctgaat ta
//
