LOCUS       sequence-79587-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRipTAL plasmid for cloning of megaTALs or other TAL-fusions and 
            expression in mammalian cells. RVDs are cloned between Age-Xho sites
            and meganuclease/fusion is cloned between Xba-Sal sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCVL RipTAL(Nd154C63) SFFV Age-Xho/Xba-Sal lin...
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(447..467)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(471..497)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(504..557)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ggccggctca gctgggccac aatgctctcg aggtcagtca gctagcatgc accggtaagc
       61 ttccttcgca gcctatacag ggcttggatt gctggccgct ctccgtactg ggccactgtg
      121 gcgatctggg aggcggacag ccgcagcggg gcggcagtaa gtgcggtagc cacaggcagc
      181 agggcttgga gcgccagatc tccgctccgc tgccgggcga tatccactat gtgagcgcga
      241 gtcagttctg gcagggcggc ggcgagctcg gggtagttcc gagcgaccac gcgcaaactc
      301 tgtctccgcc gagaaattct gcagatatca gcgtgagtaa aaccatgtcc gaccaaggcc
      361 tcatgatgct gggcaaccgt gcttcgcact ttaggcttaa ttttttcctg ctgctgctgt
      421 gaatagccga gggttctgag gtctacgact ttgcgtttct tcttaggtgg cgcataatct
      481 gggacatcat atggatacct gcaggggccc ggattctcct ccacgtcacc gcatgttaga
      541 agacttcctc tgccctctga attaagcttg tgccccagtt tgctagggag gtcgcagtat
      601 ctggccactg ccacctcgtg ctgctcgacg taggtctcgt tgttggcctc cttgattctt
      661 tccagtctgt agtccacata gtagacgcca ggcatcttga ggttcttagc gggtttcttg
      721 gatctatatg tggtcttgat gtttgcgatc agatggctcc cgcccacgag cttcagggcc
      781 atgtcgtttc tgccttccag gccgccgtca gcggggtaca gtgtctcggt gaaggcctcc
      841 cagccgagtg ttttcttctg catcacaggg ccgttggatg tgaagttcac ccctctgatc
      901 ttgacgttgt agatgaggca gccgtcctgg aggctggtgt cctgggtagc ggtcagcacg
      961 cccccgtctt c
//
