LOCUS       sequence-79589-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRipTAL plasmid for cloning of megaTALs or other TAL-fusions and 
            expression in mammalian cells. RVDs are cloned between Age-Xho sites
            and meganuclease/fusion is cloned between Xba-Sal sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCVL RipTAL(Nd154C63) SFFV Age-Xho/Xba-Sal lin...
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..111
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             118..144
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             148..168
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct taattcagag
       61 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atccgggccc ctgcaggtat
      121 ccatatgatg tcccagatta tgcgccacct aagaagaaac gcaaagtcgt agacctcaga
      181 accctcggct attcacagca gcagcaggaa aaaattaagc ctaaagtgcg aagcacggtt
      241 gcccagcatc atgaggcctt ggtcggacat ggttttactc acgctgatat ctgcagaatt
      301 tctcggcgga gacagagttt gcgcgtggtc gctcggaact accccgagct cgccgccgcc
      361 ctgccagaac tgactcgcgc tcacatagtg gatatcgccc ggcagcggag cggagatctg
      421 gcgctccaag ccctgctgcc tgtggctacc gcacttactg ccgccccgct gcggctgtcc
      481 gcctcccaga tcgccacagt ggcccagtac ggagagcggc cagcaatcca agccctgtat
      541 aggctgcgaa ggaagcttac cggtgcatgc tagctgactg acctcgagag cattgtggcc
      601 cagctgagcc ggcctgatcc ggcgttggcc gcgttgacca acgaccacct cgtcgccttg
      661 gcctgcctcg gcggacgtcc tgccatggat gcagtgaaaa agggattgcc gcacgcgccg
      721 gaattgatca gaagagtcaa tcgccgtatt ggcgaacgca cgtcccatcg cgttgcgata
      781 tctagatgac gtagtgatct gatctcagtc gaccgagcat cttaccgcca tttataccca
      841 tatttgttct gtttttcttg atttgggtat acatttaaat gttaatagaa caaaatggtg
      901 gggcaatcat ttacattttt agggatatgt aattactagt tcaggtgtat tgccacaaga
      961 caaacatgtt aagaaacttt cccgttattt acg
//
