LOCUS       sequence-79597-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Pichia pastoris expression vector with AOX1 promoter and 
            C-terminal 3C protease cleavage site and 10x His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pICXC3H
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..67)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(83..112)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(123..152)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(153..176)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             203..223
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             227..247
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    266..390
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(415..420,421..438,439..445)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(565..584)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agtgcccaac ttgaactgag gaacagtcat gtctaaggct acaaactcaa tgatgatgat
       61 gatgatggtc gacggcgcta ttcagatcct cttctgagat gagtttttgt tctagattat
      121 taatgatgat gatgatggtg atgatgatgg tgtggacctt gaaacaaaac ttctaatgca
      181 gaagagctga actagtggat ccccaaaaaa gaagagaaag gtagatccaa aaaagaagag
      241 aaaggtagct agcgaattcg atatcacaag tttgtacaaa aaagctgaac gagaaacgta
      301 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
      361 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca
      421 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
      481 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
      541 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
      601 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
      661 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
      721 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
      781 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
      841 cagtttctac acatatattc gcaagatgtg gcgtg
//
