LOCUS       sequence-79598-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Pichia pastoris expression vector with AOX1 promoter and 
            C-terminal 3C protease cleavage site, GFP and 10x His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pICXC3GH
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..67)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(83..112)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(120..149)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 agtgcccaac ttgaactgag gaacagtcat gtctaaggct acaaactcaa tgatgatgat
       61 gatgatggtc gacggcgcta ttcagatcct cttctgagat gagtttttgt tctagattaa
      121 tgatggtgat gatgatggtg atgatgatga ctagttttgt acaattcatc cataccatgg
      181 gtaataccag cagcagtaac aaattctaac aagaccatgt ggtctctctt ttcgtttgga
      241 tctttggata aggcagattg agtggataag taatggttgt ctggtaacaa gactggacca
      301 tcaccaattg gagtattttg ttgataatgg tcagctaatt gaacagaacc atcttcaatg
      361 ttgtgtctaa ttttgaagtt aactttgata ccattctttt gtttgtcagc catgatgtaa
      421 acattgtgag agttatagtt gtattccaat ttgtgaccta aaatgttacc atcttcttta
      481 aaatcaatac cttttaattc gattctatta actaaggtat caccttcaaa cttgacttca
      541 gctctggtct tgtagttacc gtcatctttg aaaaaaatag ttctttcttg aacataacct
      601 tctggcatgg cagacttgaa aaagtcatgt tgtttcatat gatctgggta tctcgcaaaa
      661 cattgaacac cataaccgaa agtagtgact aaggttggcc atggaactgg caatttacca
      721 gtagtacaaa taaattttaa ggtcaattta ccgtaagtag catcaccttc accttcaccg
      781 gagacagaaa atttgtgacc attaacatca ccatctaatt caaccaaaat tgggac
//
