LOCUS       sequence-79622-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Valentini SR, Casolari JM, Oliveira CC, Silver PA, McBride AE
  TITLE     Genetic interactions of yeast eukaryotic translation initiation 
            factor 5A (eIF5A) reveal connections to poly(A)-binding protein and 
            protein kinase C signaling.
  JOURNAL   Genetics. 2002 Feb . 160(2):393-405.
  PUBMED    11861547
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2002 Feb . 160(2):393-405."
COMMENT     Sequence Label: pPS1597
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             92..895
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg
       61 cacagaacaa aaaccgaaac gaagataaat catgtcgaaa gctacatata aggaacgtgc
      121 tgctactcat cctagtcctg ttgctgccaa gctatttaat atcatgcacg aaaagcaaac
      181 aaacttgtgt gcttcattgg atgttcgtac caccaaggaa ttactggagt tagttgaagc
      241 attaggtccc aaaatttgtt tactaaaaac acatgtggat atcttgactg atttttccat
      301 ggagggcaca gttaagccgc taaaggcatt atccgccaag tacaattttt tactcttcga
      361 agacagaaaa tttgctgaca ttggtaatac agtcaaattg cagtactctg cgggtgtata
      421 cagaatagca gaatgggcag acattacgaa tgcacacggt gtggtgggcc caggtattgt
      481 tagcggtttg aagcaggcgg cagaagaagt aacaaaggaa cctagaggcc ttttgatgtt
      541 agcagaattg tcatgcaagg gctccctatc tactggagaa tatactaagg gtactgttga
      601 cattgcgaag agcgacaaag attttgttat cggctttatt gctcaaagag acatgggtgg
      661 aagagatgaa ggttacgatt ggttgattat gacacccggt gtgggtttag atgacaaggg
      721 agacgcattg ggtcaacagt atagaaccgt ggatgatgtg gtctctacag gatctgacat
      781 tattattgtt ggaagaggac tatttgcaaa gggaagggat gctaaggtag agggtgaacg
      841 ttacagaaaa gcaggctggg aagcatattt gagaagatgc ggccagcaaa actaaaaaac
      901 tgtattataa gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa tttaattata
      961 tcagttatta ccctatgcgg tgtgaaatac cgcac
//
