LOCUS       sequence-79627-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses human Flag tagged KLF6 with 4A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Lang UE, Kocabayoglu P, Cheng GZ, Ghiassi-Nejad Z, Munoz U, Vetter 
            D, Eckstein DA, Hannivoort RA, Walsh MJ, Friedman SL
  TITLE     GSK3beta phosphorylation of the KLF6 tumor suppressor promotes its 
            transactivation of p21.
  JOURNAL   Oncogene. 2012 Oct 22. doi: 10.1038/onc.2012.457.
  PUBMED    23085750
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2012 Oct 22. doi: 10.1038/onc.2012.457."
COMMENT     Sequence Label: flag-hKLF6-4A (1010)
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             13..36
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gccgccacca tggattacaa ggacgacgac gataagatct gtcgacggta ccccggggaa
       61 ttcgcccttg ggctccgtcc aacccggccc gacatggacg tgctccccat gtgcagcatc
      121 ttccaggagc tccagatcgt gcacgagacc ggctacttct cggcgctgcc gtctctggag
      181 gagtactggc aacagacctg cctagagctg gaacgttacc tccagagcga gccctgctat
      241 gtttcagcct cagaaatcaa atttgacagc caggaagatc tgtggaccaa aatcattctg
      301 gctcgggaga aaaaggagga atccgaactg aagatatctt ccagtcctcc agaggacact
      361 ctcatcagcc cgagcttttg ttacaactta gagaccaaca gcctgaactc agatgtcagc
      421 agcgaatcct ctgacagctc cgaggaactt tctcccacgg ccaagtttac ctccgacccc
      481 attggcgaag ttttggtcag ctcgggaaaa ttgagctccg ctgtcacctc cgcgcctcca
      541 tctgctccgg aactggccag ggaaccttct caactgtggg gttgcgtgcc cggggagctg
      601 ccctcgccag ggaaggtgcg cagcgggact tcggggaagc caggtgacaa gggaaatggc
      661 gatgcctccc ccgacggcag gaggagggtg caccggtgcc actttaacgg ctgcaggaaa
      721 gtttacacca aaagctccca cttgaaagca caccagcgga cgcacacagg agaaaagcct
      781 tacagatgct catgggaagg gtgtgagtgg cgttttgcaa gaagtgatga gttaaccagg
      841 cacttccgaa agcacaccgg ggccaagcct tttaaatgct cccactgtga caggtgtttt
      901 tccaggtctg accacctggc cctgcacatg aagaggc
//
