LOCUS       sequence-79643-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NS module plasmid for Cermak, et al., 2011 TALEN kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  TITLE     module plasmid for Cermak, et al., 2011 TALEN kit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pNS8
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(187..192,193..209,210..215)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(615..634)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
       61 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      121 ttctttcctg cgttatcccc tgattctgtg gataacgcag gaaagaacat gaattaattc
      181 tcatgtttga cagcttatca tcgattagct ttaatgcggt agtttatcac agttaaattg
      241 ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac
      301 cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg
      361 ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc
      421 gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg
      481 cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac
      541 acccgtcctg tggattctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
      601 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
      661 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
      721 gttgggcgcc atctccttac atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
      781 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgcc gacccatgcc
      841 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
      901 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg
      961 ggtcattttc ggcgaggacc gctttc
//
