LOCUS       sequence-79644-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NS module plasmid for Cermak, et al., 2011 TALEN kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  TITLE     module plasmid for Cermak, et al., 2011 TALEN kit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pNS1
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(369..374,375..391,392..397))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(604..623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagagaat ccacaggacg ggtgtggtcg ccatgatcgc
       61 gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc
      121 ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag
      181 cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag
      241 taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat
      301 gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa
      361 actaccgcat taaagctaat cgatgataag ctgtcaaaca tgagaattaa ttcatgttct
      421 ttcctgcgtt atcccctgat tctgtggata acgcaggaaa gaacatgtga gcaaaaggcc
      481 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      541 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      601 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      661 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      721 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      781 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      841 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      901 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      961 gaagaacagt att
//
