LOCUS       sequence-79685-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photoconverible Fluorescent Protein_ Fixative Resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin
            JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, 
            Wang Y, Looger LL
  TITLE     Fixation-resistant photoactivatable fluorescent proteins for CLEM.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225.
  PUBMED    25581799
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225."
COMMENT     Sequence Label: pRSETa_ mEos4a
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..34
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             38..70
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(74..82,83..97)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             113..796
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     primer_bind     complement(859..877)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 acatatgcgg tgttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca
       61 gcaaatgggt cgggatctgt acgacgatga cgataaggat cgatggggat ccatggtgag
      121 tgcgattaag ccagacatga ggatcaaact ccgtatggaa ggcaacgtaa acgggcacca
      181 ctttgtgatc gacggagatg gtacaggcaa gccttatgag ggaaaacaga ccatggatct
      241 tgaagtcaaa gagggcggac ctctgccttt tgcctttgat atcctgacca ctgcattcca
      301 ttacggcaac agggtattcg ttaaatatcc agacaacata caagactatt ttaagcagtc
      361 gtttcctaag gggtattcgt gggaacgaag cttgactttc gaagacgggg gcatttgcta
      421 tgccagaaac gacataacaa tggaagggga cactttctat aataaagttc gattttatgg
      481 taccaacttt cccgccaatg gtccagttat gcagaagaag acgctgaaat gggagccctc
      541 cactgagaaa atgtatgtgc gtgatggagt gctgacgggt gatattcata tggctttgtt
      601 gcttgaagga aatgcccatt accgatgtga cttcagaact acttacaaag ctaaggagaa
      661 gggtgtcaag ttaccaggct accactttgt ggaccacgcc attgagattt taagccatga
      721 caaagattac aacaaggtta agctgtatga gcatgctgtt gctcattctg gattgcctga
      781 caatgccaga cgataagaat tcgaagcttg atccggctgc taacaaagcc cgaaaggaag
      841 ctgagttggc tgctgccacc gctgagcaat aactagcata accc
//
