LOCUS       sequence-79687-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photoconverible Fluorescent Protein_ Fixative Resistant (monomeric) 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin
            JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, 
            Wang Y, Looger LL
  TITLE     Fixation-resistant photoactivatable fluorescent proteins for CLEM.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225.
  PUBMED    25581799
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225."
COMMENT     Sequence Label: pRSETa_ mEos4b
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(70..78,79..93)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             109..792
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     primer_bind     complement(855..873)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 atgcggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
       61 atgggtcggg atctgtacga cgatgacgat aaggatcgat ggggatccat ggtgagtgcg
      121 attaagccag acatgaggat caaactccgt atggaaggca acgtaaacgg gcaccacttt
      181 gtgatcgacg gagatggtac aggcaagcct tatgagggaa aacagaccat ggatcttgaa
      241 gtcaaagagg gcggacctct gccttttgcc tttgatatcc tgaccactgc attccattac
      301 ggcaacaggg tattcgtgaa atatccagac aacatacaag actattttaa gcagtcgttt
      361 cctaaggggt attcgtggga acgaagcttg actttcgaag acgggggcat ttgcaatgcc
      421 agaaacgaca taacaatgga aggggacact ttctataata aagttcgatt ttatggtacc
      481 aactttcccg ccaatggtcc agttatgcag aagaagacgc tgaaatggga gccctccact
      541 gagaaaatgt atgtgcgtga tggagtgctg acgggtgata ttgagatggc tttgttgctt
      601 gaaggaaatg cccattaccg atgtgacttc agaactactt acaaagctaa ggagaagggt
      661 gtcaagttac caggcgccca ctttgtggac cacgccattg agattttaag ccatgacaaa
      721 gattacaaca aggttaagct gtatgagcat gctgttgctc attctggatt gcctgacaat
      781 gccagacgat aagaattcga agcttgatcc ggctgctaac aaagcccgaa aggaagctga
      841 gttggctgct gccaccgctg agcaataact agcataacc
//
