LOCUS       sequence-79689-e         539 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 E670C mutant with an N-terminal 3xFLAG-YFP tag
            in mammalian cells. cDNA is synthetic, containing convenient 
            restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 539)
  TITLE     Forbush Lab human NKCC1 NT clones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 E670C (NT473)
FEATURES             Location/Qualifiers
     source          1..539
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..20
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(75..96)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(336..355)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acaaggatga cgatgacaag ggtgacccta ccgtcagcaa gggcgaggag ctgttcaccg
       61 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      121 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      181 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgatgt
      241 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      301 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      361 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      421 tcaaggagga cggcaacatc ctagggcaca agctggagta caactacaac agccacaacg
      481 tttatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccac
//
