LOCUS       sequence-79691-e         231 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 G719C C723S C724V mutant with an N-terminal 
            3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing 
            convenient restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 231)
  TITLE     Forbush Lab human NKCC1 NT clones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 G719C C723S C724V (NT...
FEATURES             Location/Qualifiers
     source          1..231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             18..83
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(138..159)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cggccgccac catgggagac tacaaagacc atgacggtga ttataaagat cacgacatcg
       61 actacaagga tgacgatgac aagggtgacc ctaccgtcag caagggcgag gagctgttca
      121 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      181 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag t
//
