LOCUS       sequence-79711-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RALB-V23/R47 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Simicek M, Lievens S, Laga M, Guzenko D, Aushev VN, Kalev P, Baietti
            MF, Strelkov SV, Gevaert K, Tavernier J, Sablina AA
  TITLE     The deubiquitylase USP33 discriminates between RALB functions in 
            autophagy and innate immune response.
  JOURNAL   Nat Cell Biol. 2013 Oct;15(10):1220-30. doi: 10.1038/ncb2847. Epub 
            2013 Sep 22.
  PUBMED    24056301
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2847"; 
            journalName: "Nat Cell Biol"; date: "2013-10"; volume: "15"; issue: 
            "10"; pages: "1220-30"
COMMENT     Sequence Label: pLA CMV N-Flag RALB V23 R47
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..96
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(798..818)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac
       61 tctagcacca tggattacaa ggacgacgac gataagtcta gagctgccaa caagagtaag
      121 ggccagagct ccttggccct ccacaaggtg atcatggttg gcagcgtagg cgttggcaag
      181 tcagccctga cgcttcagtt catgtatgac gagtttgtag aagactatga acctaccaga
      241 gctgacagtt atagaaagaa agtggttctt gatggggaag aagttcagat agatattctg
      301 gacaccgctg ggcaagagga ctacgcagcc attcgagata actactttcg gagtggggaa
      361 gggtttcttc ttgtgttctc aatcacagaa catgaatcct ttacagcaac agccgaattc
      421 agggaacaga ttctccgtgt gaaggctgaa gaagataaaa ttccactgct cgtcgtggga
      481 aacaagtctg acctagagga gcggaggcag gtgcctgtgg aggaggccag gagtaaagcc
      541 gaagagtggg gcgtgcagta cgtggagacg tcagcgaaga cccgggccaa cgtggacaag
      601 gtgttctttg acctaatgag agaaatcaga acaaagaaga tgtcagaaaa caaagacaag
      661 aatggcaaga aaagcagcaa gaacaagaaa agttttaaag aaagatgttg cttactatga
      721 ctcgaggaat tcggatccgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
      781 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
      841 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttg
//
