LOCUS       sequence-79716-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains GFP21, which has a sequence similar to YFP, 
            but emission/excitation similar to GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Tamura M, Gu J, Matsumoto K, Aota S, Parsons R, Yamada KM
  TITLE     Inhibition of cell migration, spreading, and focal adhesions by 
            tumor suppressor PTEN.
  JOURNAL   Science. 1998 Jun 5;280(5369):1614-7.
  PUBMED    9616126
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-06-5"; volume: "280(5369)"; pages: "1614-7"
COMMENT     Sequence Label: pGFP-PTEN C124A
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        202..220
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     202..219
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     233..252
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      298..307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(304..306,307..309,310..822)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVDGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(349..370)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(610..629)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg
       61 ccgggaacgg tgcattggaa cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta
      121 tagagtctat aggcccaccc ccttggcttc gttagaacgc ggctacaatt aatacataac
      181 cttatgtatc atacacatac gatttaggtg acactataga ataacatcca ctttgccttt
      241 ctctccacag gtgtccactc ccaggtccaa ctgcacctcg gttctatcga ttcagccgcc
      301 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      361 gacggcgacg tagacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      421 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      481 accctcgtga ccaccttcgc ctacggcctg cagtgcttcg cccgctaccc cgaccacatg
      541 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      601 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      661 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      721 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
      781 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
      841 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      901 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
//
