LOCUS       sequence-79717-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Alu, a human SINE, using the pol III enhancer sequence of 
            the 7SL gene and tagged with the self splicing intron neo cassette .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Kroutter EN, Belancio VP, Wagstaff BJ, Roy-Engel AM
  TITLE     The RNA polymerase dictates ORF1 requirement and timing of LINE and 
            SINE retrotransposition.
  JOURNAL   PLoS Genet. 2009 Apr;5(4):e1000458. doi: 
            10.1371/journal.pgen.1000458. Epub 2009 Apr 24.
  PUBMED    19390602
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2009-04"; volume: "5(4):e1000458. doi"; pages: " 
            10.1371/journal.pgen.1000458. Epub 2009 Apr 24"
COMMENT     Sequence Label: pAluYa5-neo-TET
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(93..112)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gaggaggctt ttttggaggt cgagccaata tgggatcggc cattgaacaa gatggattgc
       61 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
      121 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
      181 ttgtcaagac cgacctgtcc ggtgccctga atgaactcga gtactccaaa cctaatcaat
      241 atactttcgc atacaaatta ggtcccagcg gctccagtgt tgcatcactt catccgctag
      301 ctcccattaa ggagaggtcc gactatatct tatgagaaga atacatcttc cccgaccgac
      361 atttagtctg tgaactgcat ccatatcaac agaagatctg ttgacttagg acttggctgc
      421 gtggttagga ccatgtccgt cagcttatta ccataccctt tgcaaggcca tctcaaagtt
      481 tcccctgaga cttggtactg aacggctgtt gacccctttc ccgcaatttg acggtcttgc
      541 cttttaaacc gatgcaatct attggtttaa agactagcta ccaggtgcat gcctgataac
      601 ttttccctgc aattcattat attgctaatt acaggacgag gcagcgcggc tatcgtggct
      661 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
      721 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
      781 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
      841 ctgcccattc gaccaccaag cgaaacatcg c
//
