LOCUS       sequence-79718-e         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains GFP21, which has a sequence similar to YFP, 
            but emission/excitation similar to GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Tamura M, Gu J, Matsumoto K, Aota S, Parsons R, Yamada KM
  TITLE     Inhibition of cell migration, spreading, and focal adhesions by 
            tumor suppressor PTEN.
  JOURNAL   Science. 1998 Jun 5;280(5369):1614-7.
  PUBMED    9616126
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-06-5"; volume: "280(5369)"; pages: "1614-7"
COMMENT     Sequence Label: pGFP-PTEN
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        246..264
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     246..263
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     277..296
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      342..351
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(348..350,351..353,354..866)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(393..414)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(654..673)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaccgt cagatcgcct ggagacgcca tccacgctgt
       61 tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccggga acggtgcatt
      121 ggaacgcgga ttccccgtgc caagagtgac gtaagtaccg cctatagagt ctataggccc
      181 acccccttgg cttcgttaga acgcggctac aattaataca taaccttatg tatcatacac
      241 atacgattta ggtgacacta tagaataaca tccactttgc ctttctctcc acaggtgtcc
      301 actcccaggt ccaactgcac ctcggttcta tcgattcagc cgccaccatg gtgagcaagg
      361 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      421 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      481 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct
      541 tcgcctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
      601 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      661 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      721 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      781 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
      841 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      901 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      961 agtccgccct gagcaaagac cccaacgag
//
