LOCUS       sequence-79719-e         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template DNA for in vitro transcription using T7 polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Fukaya T, Tomari Y
  TITLE     PABP is not essential for microRNA-mediated translational repression
            and deadenylation in vitro.
  JOURNAL   EMBO J. 2011 Nov 25;30(24):4998-5009. doi: 10.1038/emboj.2011.426.
  PUBMED    22117217
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011"; journalName: "EMBO J"; date: "2011-11-25- 25";
            volume: "30"; issue: "24"; pages: "4998-5009"
COMMENT     Sequence Label: pUC57-Rluc-let-7-A114-N40
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(120..224)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     292..310
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(348..370)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     470..489
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     683..705
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     697..714
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     698..714
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        727..745
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      749..758
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
       61 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
      121 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
      181 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
      241 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
      301 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
      361 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
      421 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
      481 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
      541 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
      601 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
      661 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc
      721 gagctctaat acgactcact ataggctagc caccatggct tccaaggtgt acgaccccga
      781 gcaacgcaaa cgcatgatca ctgggcctca gtggtgggct cgctgcaagc aaatgaacgt
      841 gctggactcc ttcatcaact actatgattc cgagaagcac gccgagaacg ccgtgatttt
      901 tctgcatggt aacgctgcct ccagctacct gtggaggcac gtcgtgcctc acatcgagcc
      961 cgtggctaga tgcatcatcc ctgatctga
//
