LOCUS       sequence-79721-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Self-inactivating retrovirus vector containing the DsRed-Express2 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Fujita T, Fujii H
  TITLE     Identification of Proteins Associated with an IFNgamma-Responsive 
            Promoter by a Retroviral Expression System for enChIP Using CRISPR.
  JOURNAL   PLoS One. 2014 Jul 22;9(7):e103084. doi: 
            10.1371/journal.pone.0103084. eCollection 2014.
  PUBMED    25051498
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-22"; volume: "9(7):e103084. doi"; pages: " 
            10.1371/journal.pone.0103084. eCollection 2014"
COMMENT     Sequence Label: pSIR-DsRed-Express2
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(163..840)
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(225..248)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(423..442)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     640..660
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 aaatctttta ttttatctat ggctcgtact ctataggctt cagctggtga tattgttgag
       61 tcaaaactag agcctggacc actgatatcc tgtctttaac aaattggact aatcgcggga
      121 tctccagcat gagatccccg cgctggagga tcatccagcc atctactgga acaggtggtg
      181 gcgggcctcg gcgcgctcgt actgctccac cacggtgtag tcctcgttgt gggaggtgat
      241 gtccagcttg gagtccacgt agtagtagcc gggcagcttc acgggcttct tggccatgta
      301 gattgacttg aactccacca ggtagtggcc gccgcccttc agcttcagcg ccttgtggat
      361 ctcgcccttc agcacgccgt cgcgggggta caggcgctcg gtggagggct cccagcccag
      421 agtcttcttc tgcattacgg ggccgtcgga ggggaagttc acgccgatga acttcacgtg
      481 gtagatgaag gtgccgtcct gcagggagga gtcctgggtc acggtcacca cgccgccgtc
      541 ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct tcttgtagtc
      601 ggggatgtcg gcggggtgct tcacgtacac cttggagccg tactggaact ggggggacag
      661 gatgtcccag gcgaagggca gggggccgcc cttggtcacc tgcagcttgg cggtctgggt
      721 gccctcgtag ggcttgccct cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga
      781 gccctccatg tgcaccttga agcgcatgaa gggcttgatg acgttctcag tgctatccat
      841 ggttggatca gatcgatctt gatcccctgc gccatcagat ccttggcggc aagaaa
//
