LOCUS       sequence-79726-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human eIF2C1 mutant in mamalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K
  TITLE     Short-interfering-RNA-mediated gene silencing in mammalian cells 
            requires Dicer and eIF2C translation initiation factors.
  JOURNAL   Curr Biol. 2003 Jan 8;13(1):41-6.
  PUBMED    12526743
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2003-01-8"; volume: "13(1)"; pages: "41-6"
COMMENT     Sequence Label: pcDNAM-human eIF2C1-B-Myc
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagtta agcttggtac cgagctcgga
      121 tccaccatgg agcctatgtt ccggcatctc aagaacacct actcagggct gcagctcatt
      181 attgtcatcc tgccagggaa gacgccggtg tatgctgagg tgaaacgtgt cggagataca
      241 ctcttgggaa tggctacgca gtgtgtgcag gtgaagaacg tggtcaagac ctcacctcag
      301 actctgtcca acctctgcct caagatcaat gtcaaacttg gtggcattaa caacatccta
      361 gtcccacacc agcgctctgc cgtttttcaa cagccagtga tattcctggg agcagatgtt
      421 acacaccccc cagcagggga tgggaaaaaa ccttctatca cagcagtggt aggcagtatg
      481 gatgcccacc ccagccgata ctgtgctact gtgcgggtac agcgaccacg gcaagagatc
      541 cttgaagact tgtcctacat ggtgcgtgag ctcctcatcc aattctacaa gtccacccgt
      601 ttcaagccta cccgcatcat cttctaccga gatggggtgc ctgaaggcca gctaccccag
      661 atactccact atgagctact ggccattcgt gatgcctgca tcaaactgga aaaggactac
      721 cagcctggta tcacttatat tgtggtgcag aaacgccatc acacccgcct tttctgtgct
      781 gacaagaatg agcgaattgg gaagagtggt aacatcccag ctgggaccac agtggacacc
      841 aacatcaccc acccatttga gtttgacttc tatctgtgca gccacgcagg catccagggc
      901 accagccgac catcccatta ctatgttctt tgggatgaca accgtttcac agcagatgag
      961 ctccagatcc tgacgtacca gctgtgccac acttacg
//
