LOCUS       sequence-79734-e         581 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses human cofilin R21Q mutant fused to mRFP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Mi J, Shaw AE, Pak CW, Walsh KP, Minamide LS, Bernstein BW, Kuhn TB,
            Bamburg JR
  TITLE     A genetically encoded reporter for real-time imaging of 
            cofilin-actin rods in living neurons.
  JOURNAL   PLoS One. 2013 Dec 31;8(12):e83609. doi: 
            10.1371/journal.pone.0083609.
  PUBMED    24391794
REFERENCE   2  (bases 1 to 581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-31"; volume: "8(12):e83609. doi"; pages: " 
            10.1371/journal.pone.0083609"
COMMENT     Sequence Label: pmRFP-N1 human cofilin R21Q
FEATURES             Location/Qualifiers
     source          1..581
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(132..155)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(330..349)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ctagagtcgc
       61 ggccaattct taggcgccgg tggagtggcg gccctcggcg cgctcgtact gttccacgat
      121 ggtgtagtcc tcgttgtggg aggtgatgtc cagcttgatg tcggtcttgt aggcgccggg
      181 cagctgcacg ggcttcttgg ccatgtaggt ggtcttgacc tcggcgtcgt agtggccgcc
      241 gtccttcagc ttcagcctca tcttgatctc gcccttcagg gcgccgtcct cggggtacat
      301 ccgctcggtg gaggcctccc agcccatggt cttcttctgc attacggggc cgtcggaggg
      361 gaagttggtg ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca gggaggagtc
      421 ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc acttgaagcc
      481 ctcggggaag gacagcttca agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt
      541 ggagccgtac tggaactgag gggacaggat gtcccaggcg a
//
